Expression profile of a PAL gene from Astragalus membranaceus var.   Mongholicus and its crucial role in flux into flavonoid biosynthesis

doi:10.1007/s00299-005-0072-7

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	Expression profile of a PAL gene from Astragalus membranaceus var. Mongholicus and its crucial role in flux into flavonoid biosynthesis
	Liu, RR ; Xu, SH ; Li, JL ; Hu, YL ; Lin, ZP
刊名	plant cell reports
	2006
关键词	phenylalanine ammonia-lyase Astragalus membranaceus var. Mongholicus expression profile flavonoid biosynthesis PHENYLALANINE AMMONIA-LYASE RADIX-ASTRAGALI METABOLISM LETTUCE INVOLVEMENT PARSLEY PATHWAY ENZYMES LEAVES PLANTS
DOI	10.1007/s00299-005-0072-7
英文摘要	An important traditional Chinese medicine herb, Astragalus membranaceus var. Mongholicus, whose dried root is known as Radix astragali ("Huangqi" in Chinese), has high flavonoid content as an essential active constituent. Phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) catalyzes the first and also a rate-limiting step in phenylpropanoid pathway, which supplies precursors for a variety of secondary metabolites including flavonoids. A PAL gene, designated AmPAL1 (GenBank accession no. AY986506), was isolated from A. membranaceus var. Mongholicus with a full-length cDNA of 2562 nucleotides and an open reading frame of 2154 bp. Northern blot analysis revealed that AmPAL1 expressed universally in different organs, and its expression was markedly induced by UV irradiation, mechanical wounding, and white light irradiation on etiolated seedlings, with some distinctive responsive properties. Content of a typical flavonoid, quercetin, in A. membranaceus var. Mongholicus of different ages correlated with PAL enzymatic activity. Transgenic tobacco plants harboring AmPAL1 under the control of the CaMV35S promoter showed significantly increased PAL activity and correlatively increased quercetin content than those in non-transformed plants. These results indicate that PAL is maybe a key point for flux into flavonoid biosynthesis in the genetic control of secondary metabolism in A. membranaceus var. Mongholicus.; Plant Sciences; SCI(E); EI; PubMed; 35; ARTICLE; 7; 705-710; 25
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/199364]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu, RR,Xu, SH,Li, JL,et al. Expression profile of a PAL gene from Astragalus membranaceus var. Mongholicus and its crucial role in flux into flavonoid biosynthesis[J]. plant cell reports,2006.
APA	Liu, RR,Xu, SH,Li, JL,Hu, YL,&Lin, ZP.(2006).Expression profile of a PAL gene from Astragalus membranaceus var. Mongholicus and its crucial role in flux into flavonoid biosynthesis.plant cell reports.
MLA	Liu, RR,et al."Expression profile of a PAL gene from Astragalus membranaceus var. Mongholicus and its crucial role in flux into flavonoid biosynthesis".plant cell reports (2006).