Cloning of crucian carp (Carassius cuvieri) metallothionein-II gene and   characterization of its gene promoter region

doi:10.1016/j.bbrc.2006.02.082

CORC > 北京大学 > 生命科学学院

	Cloning of crucian carp (Carassius cuvieri) metallothionein-II gene and characterization of its gene promoter region
	Ren, HW ; Xu, MX ; He, PF ; Muto, N ; Itoh, N ; Tanaka, K ; Xing, J ; Chu, MM
刊名	生物化学与生物物理学研究通讯
	2006
关键词	metallothionein gene regulation MRE GRE Carassius cuvieri METAL-RESPONSIVE ELEMENTS REGULATORY ELEMENTS B-GENE FUNCTIONAL-ANALYSIS ZINC-METABOLISM CDNA CLONING COMMON CARP A GENE EXPRESSION FISH
DOI	10.1016/j.bbrc.2006.02.082
英文摘要	The genomic DNA of crucian carp (Carassius cuvieri) metallothiownein-II (ccMT-II), with its upstream region was obtained. The sequence analysis Of its upstream region revealed several putative cis-acting elements including seven metal regulatory elements (MREs), three activator protein 1 (AP1), two glucocorticoid response element (GREs) etc. The seven MREs locate into two clusters, a distal cluster with four MREs within -800/-600 bp from the translation start site and a proximal cluster with three MREs close to TATA box. In transient luciferase gene expression assays, both of the distal and proximal cluster MREs have significantly shown synergistic effects in the transcription of ccMT-II gene; the proximal cluster of MREs serves as the major elements in metal inducing activity; Zn2+ and Cd2+ served as much stronger inducers than Cu2+ shown in ccMT-II expression. The two GRE homologous sequences in ccMT-II promoter showed not inductive in either HepG2 or HEK293. (c) 2006 Elsevier Inc. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000236316100043&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Biophysics; SCI(E); PubMed; 15; ARTICLE; 4; 1297-1304; 342
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/199324]
专题	生命科学学院
推荐引用方式 GB/T 7714	Ren, HW,Xu, MX,He, PF,et al. Cloning of crucian carp (Carassius cuvieri) metallothionein-II gene and characterization of its gene promoter region[J]. 生物化学与生物物理学研究通讯,2006.
APA	Ren, HW.,Xu, MX.,He, PF.,Muto, N.,Itoh, N.,...&Chu, MM.(2006).Cloning of crucian carp (Carassius cuvieri) metallothionein-II gene and characterization of its gene promoter region.生物化学与生物物理学研究通讯.
MLA	Ren, HW,et al."Cloning of crucian carp (Carassius cuvieri) metallothionein-II gene and characterization of its gene promoter region".生物化学与生物物理学研究通讯 (2006).