Proteomic analysis and comparison of the biopsy and autopsy specimen of   human brain temporal lobe

doi:10.1002/pmic.200600078

CORC > 北京大学 > 生命科学学院

	Proteomic analysis and comparison of the biopsy and autopsy specimen of human brain temporal lobe
	He, Sizhi ; Wang, Qingsong ; He, Jintang ; Pu, Hai ; Yang, Wei ; Ji, Jianguo
刊名	proteomics
	2006
关键词	2-DE biopsy and autopsy specimen human brain temporal lobe MALDI-TOF-MS I INDUCES TRANSLOCATION ALZHEIMERS-DISEASE MESSENGER-RNA A-I NEUROFIBRILLARY TANGLES POSTMORTEM DELAY ALPHA-CRYSTALLIN BINDING-PROTEIN CEREBRAL-CORTEX EXPRESSION
DOI	10.1002/pmic.200600078
英文摘要	The proteomic study on human temporal lobe can help us to understand the physiological function of CNS in normal as well as in pathological state. Proteomic tools are potent for the assessment of protein stability post mortem. in this pilot study, the human temporal lobe biopsy specimen with chronic pharmacoresistant temporal lobe epilepsy (TLE) and autopsy specimen in control were separated by 2-DE. Using MALDI-TOF-MS and MS/MS, 375 protein spots were identified which were the products of 267 genes. Six down-regulated and 23 up-regulated protein spots in the autopsy specimen were ascertained after the gel image analysis with the Image-Master software. A number of proteins that include neurotransmitter metabolic and glycolytic enzymes, cytoprotective proteins and cytoskeleton were found decreased while the precursor of apolipoprotein A-I increased in the TLE brain. We tried several methods to prepare the protein samples and found that DNase and RNase treatment, ultracentrifugation and Amersham cleanup kit purification can improve gel separation quality This work optimized the sample preparation method and constructed a primary protein database of human temporal lobe and found some proteins with remarkable level change probably involved in the post-mortem process and chronic pharmacoresistant TLE pathogenesis.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000240875500010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; Biochemistry & Molecular Biology; SCI(E); PubMed; 12; ARTICLE; 18; 4987-4996; 6
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198978]
专题	生命科学学院
推荐引用方式 GB/T 7714	He, Sizhi,Wang, Qingsong,He, Jintang,et al. Proteomic analysis and comparison of the biopsy and autopsy specimen of human brain temporal lobe[J]. proteomics,2006.
APA	He, Sizhi,Wang, Qingsong,He, Jintang,Pu, Hai,Yang, Wei,&Ji, Jianguo.(2006).Proteomic analysis and comparison of the biopsy and autopsy specimen of human brain temporal lobe.proteomics.
MLA	He, Sizhi,et al."Proteomic analysis and comparison of the biopsy and autopsy specimen of human brain temporal lobe".proteomics (2006).