Production of soybean isoflavone genistein in non-legume plants via genetically modified secondary metabolism pathway | |
Liu, Rongrong ; Hu, Yuanlei ; Li, Halin ; Lin, Zhongping | |
刊名 | metabolic engineering
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2007 | |
关键词 | genistein metabolic engineering isollavone synthase flavanone 3-hydroxylase phenylalanine ammonia-lyase PHENYLALANINE AMMONIA-LYASE SACCHAROMYCES-CEREVISIAE ESCHERICHIA-COLI FLAVONOID BIOSYNTHESIS FUNCTIONAL EXPRESSION TRANSGENIC TOBACCO NATURAL-PRODUCTS HEALTH ARABIDOPSIS SYNTHASE |
DOI | 10.1016/j.ymben.2006.08.003 |
英文摘要 | Genetic modification of secondary metabolic pathways to produce desirable natural products is an attractive approach in plant biotechnology. In our study, we attempted to produce a typical soybean isoflavone genistein, a well-known health-promoting metabolite, in non-legume plants via genetic engineering. Both overexpression and antisense suppression strategies were used to manipulate the expression of several genes encoding key enzymes in the flavonoids/isoflavonoids pathway in transgenic tobacco, lettuce, and petunia. Introducing soybean isoflavone synthase (IFS) into these plants, which naturally do not produce isoflavonoids due to a lack of this leguminous enzyme.. resulted in genistein biosynthesis in tobacco petals, petunia leaves and petals, and lettuce leaves. In tobacco, when flavanone 3-hydroxylase (F3H) expression was suppressed by its antisense gene while soybean IFS was overexpressed at the same time, genistein yield increased prominently. In addition, overexpression of phenylalanine ammonia-lyase (PAL) also led to an enhanced genistein production in tobacco petals and lettuce leaves in the presence of IFS than in the plants that overexpressed only IFS. (C) 2006 Elsevier Inc. All rights reserved.; Biotechnology & Applied Microbiology; SCI(E); EI; PubMed; 33; ARTICLE; 1; 1-7; 9 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/198672] ![]() |
专题 | 生命科学学院 |
推荐引用方式 GB/T 7714 | Liu, Rongrong,Hu, Yuanlei,Li, Halin,et al. Production of soybean isoflavone genistein in non-legume plants via genetically modified secondary metabolism pathway[J]. metabolic engineering,2007. |
APA | Liu, Rongrong,Hu, Yuanlei,Li, Halin,&Lin, Zhongping.(2007).Production of soybean isoflavone genistein in non-legume plants via genetically modified secondary metabolism pathway.metabolic engineering. |
MLA | Liu, Rongrong,et al."Production of soybean isoflavone genistein in non-legume plants via genetically modified secondary metabolism pathway".metabolic engineering (2007). |
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