Serial nuclear transfer improves the developmental potential of mouse   embryos cloned from oocytes matured in a protein-free medium

doi:10.1002/mrd.20614

CORC > 北京大学 > 生命科学学院

	Serial nuclear transfer improves the developmental potential of mouse embryos cloned from oocytes matured in a protein-free medium
	Bai, Zhaodai ; Yong, Yun ; Qing, Tingting ; Cheng, Jing ; Shen, Wei ; Ding, Mingxiao ; Deng, Hongkui
刊名	分子繁殖和发展
	2007
关键词	germinal vesicle (GV) oocyte protein-free medium serial nuclear transfer mouse IN-VITRO FERTILIZATION MALE PRONUCLEUS FORMATION EPIDERMAL-GROWTH-FACTOR CUMULUS CELL-NUCLEI TAIL-TIP CELLS PIG OOCYTES BOVINE OOCYTES PORCINE OOCYTES SOMATIC-CELLS ENUCLEATED OOCYTES
DOI	10.1002/mrd.20614
英文摘要	Germinal vesicle (GV) oocytes matured in vitro are an alternative source for cytoplasmic recipients of nuclear transfer (NT). However, the developmental potential of oocytes matured in vitro is limited. In this study, we developed a protein-free maturation medium for mouse GV oocytes. Following parthenogenetic activation, the oocytes matured in the protein-free medium develop to blastocyst stage with a high efficiency, even up to the rate obtained from in vivo MII-oocytes (90.6% vs. 92.8%). Using the oocytes matured in the protein-free medium as the recipient, NT embryos develop to the blastocyst stage (17.6%). To further improve the developmental potential of NT embryos, we performed serial NT and compared the effect of three different activated cytoplasm samples derived from in vitro matured oocytes as the second recipient, that is, the effect of in vitro fertilized (IVF) zygote, the preactivated cytoplast and the IVF cytoplast, on the development of NT embryos. We found that when the pronucleus of NT zygote was transferred into the cytoplasm of the IVF zygote, the blastocyst formation increased to 39.4%. This is the first report to demonstrate the IVF zygote from oocytes matured in protein-free medium can be used successfully as the recipient for serial NT to enhance the developmental potential of mouse NT embryos from oocytes matured in the protein-free medium.; Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Reproductive Biology; SCI(E); PubMed; 4; ARTICLE; 5; 560-567; 74
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198668]
专题	生命科学学院
推荐引用方式 GB/T 7714	Bai, Zhaodai,Yong, Yun,Qing, Tingting,et al. Serial nuclear transfer improves the developmental potential of mouse embryos cloned from oocytes matured in a protein-free medium[J]. 分子繁殖和发展,2007.
APA	Bai, Zhaodai.,Yong, Yun.,Qing, Tingting.,Cheng, Jing.,Shen, Wei.,...&Deng, Hongkui.(2007).Serial nuclear transfer improves the developmental potential of mouse embryos cloned from oocytes matured in a protein-free medium.分子繁殖和发展.
MLA	Bai, Zhaodai,et al."Serial nuclear transfer improves the developmental potential of mouse embryos cloned from oocytes matured in a protein-free medium".分子繁殖和发展 (2007).