Directed differentiation of human embryonic stem cells into functional   hepatic cells

doi:10.1002/hep.21582

CORC > 北京大学 > 生命科学学院

	Directed differentiation of human embryonic stem cells into functional hepatic cells
	Cai, Jun ; Zhao, Yang ; Liu, Yanxia ; Ye, Fei ; Song, Zhihua ; Qin, Han ; Meng, Sha ; Chen, Yuezhou ; Zhou, Rudan ; Song, Xijun ; Guo, Yushan ; Ding, Mingxiao ; Deng, Hongkui
刊名	hepatology
	2007
关键词	HEPATOCYTE-LIKE CELLS C-VIRUS IN-VITRO LIVER DEVELOPMENT DEFINITIVE ENDODERM GROWTH-FACTORS ONCOSTATIN-M REPLICATION CD81 TRANSPLANTATION
DOI	10.1002/hep.21582
英文摘要	The differentiation capacity of human embryonic stem cells (hESCs) holds great promise for therapeutic applications. We report a novel three-stage method to efficiently direct the differentiation of human embryonic stem cells into hepatic cells in serum-free medium. Human ESCs were first differentiated into definitive endoderm cells by 3 days of Activin A treatment. Next, the presence of fibroblast growth factor-4 and bone morphogenetic protein-2 in the culture medium for 5 days induced efficient hepatic differentiation from definitive endoderm cells. Approximately 70% of the cells expressed the hepatic marker albumin. After 10 days of further in vitro maturation, these cells expressed the adult liver cell markers tyrosine aminotransferase, tryptophan oxygenase 2, phosphoenolpyruvate carboxykinase (PEPCK), Cyp7A1, Cyp3A4 and Cyp2B6. Furthermore, these cells exhibited functions associated with mature hepatocytes including albumin secretion, glycogen storage, indocyanine green, and low-density lipoprotein uptake, and inducible cytochrome P450 activity. When transplanted into CC1(4) injured severe combined immunodeficiency mice, these cells integrated into the mouse liver and expressed human alpha-1 antitrypsin for at least 2 months. In addition, we found that the hESC-derived hepatic cells were readily infected by human immunodeficiency virus-hepatitis C virus pseudotype viruses. Conclusion: We have developed an efficient way to direct the differentiation of human embryonic stem cells into cells that exhibit characteristics of mature hepatocytes. Our studies should facilitate searching the molecular mechanisms underlying human liver development, and form the basis for hepatocyte transplantation and drug tests.; Gastroenterology & Hepatology; SCI(E); PubMed; 254; ARTICLE; 5; 1229-1239; 45
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198397]
专题	生命科学学院
推荐引用方式 GB/T 7714	Cai, Jun,Zhao, Yang,Liu, Yanxia,et al. Directed differentiation of human embryonic stem cells into functional hepatic cells[J]. hepatology,2007.
APA	Cai, Jun.,Zhao, Yang.,Liu, Yanxia.,Ye, Fei.,Song, Zhihua.,...&Deng, Hongkui.(2007).Directed differentiation of human embryonic stem cells into functional hepatic cells.hepatology.
MLA	Cai, Jun,et al."Directed differentiation of human embryonic stem cells into functional hepatic cells".hepatology (2007).