Identification of proteins expressed at extremely low level in   Arabidopsis leaves

doi:10.1016/j.bbrc.2007.04.189

CORC > 北京大学 > 生命科学学院

	Identification of proteins expressed at extremely low level in Arabidopsis leaves
	Xu, Yu ; Wang, Bal-Chen ; Zhu, Yu-Xian
刊名	生物化学与生物物理学研究通讯
	2007
关键词	2-DE PolyU-affinity column arabidopsis RNA-binding protein microarray QRT-PCR RNA-BINDING PROTEINS PROTEOMIC ANALYSIS SEED-GERMINATION
DOI	10.1016/j.bbrc.2007.04.189
英文摘要	2-DE related techniques have been broadly used for protein separation and identification in recent years. However, many important cellular components often escape detection by 2-DE due to its inherent low resolution. Here we use a polyU-affinity column to enrich proteins with nucleotide-bin ding motifs. As a result, 26 enriched protein spots, including three new Arabidopsis RNA-binding proteins cp31A, cp31B, and CSP41, are identified by MALDI-TOF-MS. In the sum, 15 protein spots encoded by 12 individual genes are observed on the 2-DE only after binding to the polyU-affinity column. Two of these genes are expressed at lower than 0.1% the level of TUB4 that were considered mainly as non-expressed genes in several ATH1 microarray analysis of Arabiclopsis leaf samples. Our results suggest that polyU or similar media may be combined with 2-DE techniques to identify cellular proteins with extremely low transcription levels to fulfill specific research needs. (c) 2007 Elsevier Inc. All rights reserved.; Biochemistry & Molecular Biology; Biophysics; SCI(E); PubMed; 9; ARTICLE; 3; 808-812; 358
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198355]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xu, Yu,Wang, Bal-Chen,Zhu, Yu-Xian. Identification of proteins expressed at extremely low level in Arabidopsis leaves[J]. 生物化学与生物物理学研究通讯,2007.
APA	Xu, Yu,Wang, Bal-Chen,&Zhu, Yu-Xian.(2007).Identification of proteins expressed at extremely low level in Arabidopsis leaves.生物化学与生物物理学研究通讯.
MLA	Xu, Yu,et al."Identification of proteins expressed at extremely low level in Arabidopsis leaves".生物化学与生物物理学研究通讯 (2007).