Cloning and functional characterization of 5 &apos;-upstream region of   metallothionein-I gene from crucian carp (Carassius cuvieri)

doi:10.1016/j.biocel.2007.01.008

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	Cloning and functional characterization of 5 '-upstream region of metallothionein-I gene from crucian carp (Carassius cuvieri)
	He, Pengfei ; Xu, Mingxu ; Ren, Hongwei
刊名	international journal of biochemistry cell biology
	2007
关键词	metallothionein gene regulation metal regulatory element antioxidant response element crucian carp TRANSCRIPTION FACTOR MTF-1 ANTIOXIDANT RESPONSE ELEMENT METAL REGULATORY ELEMENTS PROMOTER REGION B-GENE FRESH-WATER A GENE EXPRESSION BIOMARKER FISH
DOI	10.1016/j.biocel.2007.01.008
英文摘要	Metallothioneins are low molecular weight, cysteine-rich, metal-binding proteins, which can be induced by heavy metal ions, cytokines, stress, and hormones. To investigate the roles of the main cis-acting elements involved in the inducible expression of metallothionein gene in fish, the 5'-upstream region of crucian carp (Carassius cuvieri) metallothionein-I gene had been cloned and analyzed after our previous work on metallothionein-II. In its upstream region, several putative cis-acting elements, including nine metal regulatory elements (MREs), one antioxidant response element, one E-box, and three interleukin-6 responsive elements, etc. were found. The nine metal regulatory elements are confined in less than 1000 bp from ATG start codon and organized into two clusters with different roles to the induction of the metallothionein-I expression. Deletion mutant assays demonstrated that both the distal and proximal clusters of metal regulatory elements contributed to the basal expression of the metallothionein-I, but only the proximal cluster was the chief contributor to the metal fold induction. In transient luciferase reporter assays, Zn2+ and Cd2+, served as much stronger inducers than Cu2+ to the metallothionein-I expression. H2O2 also could activate the metallothionein-I promoter about two-fold, which was mediated by the antioxidant response element (TGACAACGC, -437/-445). In conclusion, our studies demonstrate the roles of metal regulatory element and antioxidant response element in the induction of crucian carp metallothionein-I gene, and provide the regulatory mechanism for the use of fish metallothionein as a biomarker for monitoring of metal contamination in waters. (c) 2007 Elsevier Ltd. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000245417600018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Cell Biology; SCI(E); PubMed; 9; ARTICLE; 4; 832-841; 39
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198146]
专题	生命科学学院
推荐引用方式 GB/T 7714	He, Pengfei,Xu, Mingxu,Ren, Hongwei. Cloning and functional characterization of 5 '-upstream region of metallothionein-I gene from crucian carp (Carassius cuvieri)[J]. international journal of biochemistry cell biology,2007.
APA	He, Pengfei,Xu, Mingxu,&Ren, Hongwei.(2007).Cloning and functional characterization of 5 '-upstream region of metallothionein-I gene from crucian carp (Carassius cuvieri).international journal of biochemistry cell biology.
MLA	He, Pengfei,et al."Cloning and functional characterization of 5 '-upstream region of metallothionein-I gene from crucian carp (Carassius cuvieri)".international journal of biochemistry cell biology (2007).