Microinjection as a tool of mechanical delivery

doi:10.1016/j.copbio.2008.07.005

CORC > 北京大学 > 生命科学学院

	Microinjection as a tool of mechanical delivery
	Zhang, Yan ; Yu, Long-Chuan
刊名	生物技术新见
	2008
关键词	EMBRYONIC STEM-CELLS INTRACYTOPLASMIC SPERM INJECTION XENOPUS-LAEVIS OOCYTES HUMAN PRIMARY NEURONS PRION PROTEIN ANTISENSE OLIGONUCLEOTIDES GENE-TRANSFER ELECTROPORATION EXPRESSION BAX
DOI	10.1016/j.copbio.2008.07.005
英文摘要	Microinjection to single cells has been widely used in the studies of transduction-challenged cells, transgenic animal production, and in vitro fertilization to mechanically transfer DNAs, RNA interferences, sperms, proteins, peptides, and drugs. The advantages of microinjection include the precision of delivery dosage and timing, high efficiency of transduction as well as low cytotoxicity. However, manual microinjection is labor intensive and time consuming, which limits the application of this technique to large number of cells in a sample. New cell culture matrix ensuring all cells grow in a desired position and orientation is needed for application of high throughput automatic injection systems, which will significantly increase injection speed, cell survival, and success rates.; Biochemical Research Methods; Biotechnology & Applied Microbiology; SCI(E); PubMed; 34; REVIEW; 5; 506-510; 19
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/197574]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang, Yan,Yu, Long-Chuan. Microinjection as a tool of mechanical delivery[J]. 生物技术新见,2008.
APA	Zhang, Yan,&Yu, Long-Chuan.(2008).Microinjection as a tool of mechanical delivery.生物技术新见.
MLA	Zhang, Yan,et al."Microinjection as a tool of mechanical delivery".生物技术新见 (2008).