Role of MEK-ERK Pathway in Morphine-Induced Conditioned Place Preference   in Ventral Tegmental Area of Rats

doi:10.1002/jnr.22326

CORC > 北京大学 > 生命科学学院

	Role of MEK-ERK Pathway in Morphine-Induced Conditioned Place Preference in Ventral Tegmental Area of Rats
	Lin, XiaoJing ; Wang, QingSong ; Ji, Jianguo ; Yu, Long-Chuan
刊名	journal of neuroscience research
	2010
关键词	opioid addiction conditioned place preference (CPP) ERK morphine proteomic analysis ventral tegmental area (VTA) SENESCENCE-ACCELERATED MOUSE REGULATED PROTEIN-KINASE ELEMENT-BINDING PROTEIN PROTEOMIC ANALYSIS IN-VIVO MOLECULAR-MECHANISMS POTENTIAL PROTECTION PREFRONTAL CORTEX NUCLEUS-ACCUMBENS CORTICAL-NEURONS
DOI	10.1002/jnr.22326
英文摘要	A major goal of research on drug addiction is to develop the effective treatments to deal with the long-term behavioral disorders especially reinstatement induced by the addictive drugs such as opiates, cocaine, and cannabinoid. The molecular mechanisms underlying these substance-related disorders remain unclear so far. Here we used the model of morphine-induced conditioned place preference (CPP) in rats to mimic the progress of drug-taking, withdrawal and relapse in human. The tissue of ventral tegmental area (VTA), one of the most important brain structures associated with abused drug-related disorders, was taken and two-dimensional electrophoresis (2-DE) was performed to analyze and compare the changes of protein expression patterns during the different stages of morphine-induced CPP. First, we found that there were 80 proteins identified to be changed in the process of morphine-induced CPP. Furthermore, as the mitogen-activated protein kinase kinase 1 (MAPKK1) was increased significantly in the stages of establishment and reinstatement, we confirmed the change of activated extracellular signal-regulated kinase (ERK) by Western blotting in VTA tissue and cultured cell. The results demonstrated that the activated MEK-ERK pathway by chronic morphine treatment in VTA was involved in morphine-induced reinstatement. Moreover, inhibition of MEK-ERK pathway by infusion the MEK inhibitor U0126 in VTA blocked the establishment of morphine-induced CPP. The present study found significant changes in a group of protein expressions in VTA during morphine-induced CPP and further confirmed the role of MEK-ERK cell signaling pathway of VTA in morphine addiction. (C) 2010 Wiley-Liss, Inc.; Neurosciences; SCI(E); PubMed; 14; ARTICLE; 7; 1595-1604; 88
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/196969]
专题	生命科学学院
推荐引用方式 GB/T 7714	Lin, XiaoJing,Wang, QingSong,Ji, Jianguo,et al. Role of MEK-ERK Pathway in Morphine-Induced Conditioned Place Preference in Ventral Tegmental Area of Rats[J]. journal of neuroscience research,2010.
APA	Lin, XiaoJing,Wang, QingSong,Ji, Jianguo,&Yu, Long-Chuan.(2010).Role of MEK-ERK Pathway in Morphine-Induced Conditioned Place Preference in Ventral Tegmental Area of Rats.journal of neuroscience research.
MLA	Lin, XiaoJing,et al."Role of MEK-ERK Pathway in Morphine-Induced Conditioned Place Preference in Ventral Tegmental Area of Rats".journal of neuroscience research (2010).