Allelic Analyses of the Arabidopsis YUC1 Locus Reveal Residues and   Domains Essential for the Functions of YUC Family of Flavin   Monooxygenases

doi:10.1111/j.1744-7909.2010.01007.x

CORC > 北京大学 > 生命科学学院

	Allelic Analyses of the Arabidopsis YUC1 Locus Reveal Residues and Domains Essential for the Functions of YUC Family of Flavin Monooxygenases
	Hou, Xianhui ; Liu, Sainan ; Pierri, Florencia ; Dai, Xinhua ; Qu, Li-Jia ; Zhao, Yunde
刊名	journal of integrative plant biology
	2011
关键词	AUXIN BIOSYNTHESIS SUBSTRATE SPECIFICITIES FMO1 ORGANOGENESIS RESISTANCE MECHANISM MUTATION ENCODES ENZYMES TISSUES
DOI	10.1111/j.1744-7909.2010.01007.x
英文摘要	Flavin monooxygenases (FMOs) play critical roles in plant growth and development by synthesizing auxin and other signaling molecules. However, the structure and function relationship within plant FMOs is not understood. Here we defined the important residues and domains of the Arabidopsis YUC1 FMO, a key enzyme in auxin biosynthesis. We previously showed that simultaneous inactivation of YUC1 and its homologue YUC4 caused severe defects in vascular and floral development. We mutagenized the yuc4 mutant and screened for mutants with phenotypes similar to those of yuc1 yuc4 double mutants. Among the isolated mutants, five of them contained mutations in the YUC1 gene. Interestingly, the mutations identified in the new yuc1 alleles were concentrated in the two GXGXXG motifs that are highly conserved among the plant FMOs. One such motif presumably binds to flavin adenine dinucleotide (FAD) cofactor and the other binds to nicotinamide adenine dinucleotide phosphate (NADPH). We also identified the Ser(139) to Phe conversion in yuc1, a mutation that is located between the two nucleotide-binding sites. By analyzing a series of yuc1 mutants, we identified key residues and motifs essential for the functions of YUC1 FMO.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000285923700007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Plant Sciences; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 7; ARTICLE; 1; 54-62; 53
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/194677]
专题	生命科学学院
推荐引用方式 GB/T 7714	Hou, Xianhui,Liu, Sainan,Pierri, Florencia,et al. Allelic Analyses of the Arabidopsis YUC1 Locus Reveal Residues and Domains Essential for the Functions of YUC Family of Flavin Monooxygenases[J]. journal of integrative plant biology,2011.
APA	Hou, Xianhui,Liu, Sainan,Pierri, Florencia,Dai, Xinhua,Qu, Li-Jia,&Zhao, Yunde.(2011).Allelic Analyses of the Arabidopsis YUC1 Locus Reveal Residues and Domains Essential for the Functions of YUC Family of Flavin Monooxygenases.journal of integrative plant biology.
MLA	Hou, Xianhui,et al."Allelic Analyses of the Arabidopsis YUC1 Locus Reveal Residues and Domains Essential for the Functions of YUC Family of Flavin Monooxygenases".journal of integrative plant biology (2011).