Crystallization and preliminary X-ray analysis of argininosuccinate   lyase from Streptococcus mutans

doi:10.1107/S1744309111011377

CORC > 北京大学 > 生命科学学院

	Crystallization and preliminary X-ray analysis of argininosuccinate lyase from Streptococcus mutans
	Cao, Yan-Li ; Li, Gui-Lan ; Wang, Kai-Tuo ; Zhang, Hong-Yin ; Li, Lan-Fen
刊名	acta crystallographica section f structural biology and crystallization communications
	2011
关键词	DELTA-2 CRYSTALLIN ESCHERICHIA-COLI
DOI	10.1107/S1744309111011377
英文摘要	Argininosuccinate lyase (ASL) is an important enzyme in arginine synthesis and the urea cycle, which are highly conserved from bacteria to eukaryotes. The gene encoding Streptococcus mutans ASL (smASL) was amplified and cloned into expression vector pET28a. The recombinant smASL protein was expressed in a soluble form in Escherichia coli strain BL21 (DE3) and purified to homogeneity by two-step column chromatography. Crystals suitable for X-ray analysis were obtained and X-ray diffraction data were collected to a resolution of 2.5 angstrom. The crystals belonged to space group R3, with unit-cell parameters a = b = 254.5, c = 78.3 angstrom.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000291216800011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography; SCI(E); PubMed; 0; ARTICLE; Pt 6; 682-684; 67
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/193618]
专题	生命科学学院
推荐引用方式 GB/T 7714	Cao, Yan-Li,Li, Gui-Lan,Wang, Kai-Tuo,et al. Crystallization and preliminary X-ray analysis of argininosuccinate lyase from Streptococcus mutans[J]. acta crystallographica section f structural biology and crystallization communications,2011.
APA	Cao, Yan-Li,Li, Gui-Lan,Wang, Kai-Tuo,Zhang, Hong-Yin,&Li, Lan-Fen.(2011).Crystallization and preliminary X-ray analysis of argininosuccinate lyase from Streptococcus mutans.acta crystallographica section f structural biology and crystallization communications.
MLA	Cao, Yan-Li,et al."Crystallization and preliminary X-ray analysis of argininosuccinate lyase from Streptococcus mutans".acta crystallographica section f structural biology and crystallization communications (2011).