Crystallization and preliminary X-ray analysis of argininosuccinate lyase from Streptococcus mutans | |
Cao, Yan-Li ; Li, Gui-Lan ; Wang, Kai-Tuo ; Zhang, Hong-Yin ; Li, Lan-Fen | |
刊名 | acta crystallographica section f structural biology and crystallization communications
![]() |
2011 | |
关键词 | DELTA-2 CRYSTALLIN ESCHERICHIA-COLI |
DOI | 10.1107/S1744309111011377 |
英文摘要 | Argininosuccinate lyase (ASL) is an important enzyme in arginine synthesis and the urea cycle, which are highly conserved from bacteria to eukaryotes. The gene encoding Streptococcus mutans ASL (smASL) was amplified and cloned into expression vector pET28a. The recombinant smASL protein was expressed in a soluble form in Escherichia coli strain BL21 (DE3) and purified to homogeneity by two-step column chromatography. Crystals suitable for X-ray analysis were obtained and X-ray diffraction data were collected to a resolution of 2.5 angstrom. The crystals belonged to space group R3, with unit-cell parameters a = b = 254.5, c = 78.3 angstrom.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000291216800011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography; SCI(E); PubMed; 0; ARTICLE; Pt 6; 682-684; 67 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/193618] ![]() |
专题 | 生命科学学院 |
推荐引用方式 GB/T 7714 | Cao, Yan-Li,Li, Gui-Lan,Wang, Kai-Tuo,et al. Crystallization and preliminary X-ray analysis of argininosuccinate lyase from Streptococcus mutans[J]. acta crystallographica section f structural biology and crystallization communications,2011. |
APA | Cao, Yan-Li,Li, Gui-Lan,Wang, Kai-Tuo,Zhang, Hong-Yin,&Li, Lan-Fen.(2011).Crystallization and preliminary X-ray analysis of argininosuccinate lyase from Streptococcus mutans.acta crystallographica section f structural biology and crystallization communications. |
MLA | Cao, Yan-Li,et al."Crystallization and preliminary X-ray analysis of argininosuccinate lyase from Streptococcus mutans".acta crystallographica section f structural biology and crystallization communications (2011). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论