Four Closely-related RING-type E3 Ligases, APD1-4, are Involved in   Pollen Mitosis II Regulation in Arabidopsis

doi:10.1111/j.1744-7909.2012.01152.x

CORC > 北京大学 > 生命科学学院

	Four Closely-related RING-type E3 Ligases, APD1-4, are Involved in Pollen Mitosis II Regulation in Arabidopsis
	Luo, Guo ; Gu, Hongya ; Liu, Jingjing ; Qu, Li-Jia
刊名	journal of integrative plant biology
	2012
关键词	Male gametogenesis pollen mitosis II RING-finger E3 ligase Arabidopsis CELL-CYCLE PROGRESSION GAMETOPHYTE DEVELOPMENT UBIQUITIN LIGASES SEED-GERMINATION LEAF DEVELOPMENT GENE-EXPRESSION THALIANA DEGRADATION PROTEIN GAMETOGENESIS
DOI	10.1111/j.1744-7909.2012.01152.x
英文摘要	Ubiquitination of proteins is one of the critical regulatory mechanisms in eukaryotes. In higher plants, protein ubiquitination plays an essential role in many biological processes, including hormone signaling, photomorphogenesis, and pathogen defense. However, the roles of protein ubiquitination in the reproductive process are not clear. In this study, we identified four plant-specific RING-finger genes designated Aberrant Pollen Development 1 (APD1) to APD4, as regulators of pollen mitosis II (PMII) in Arabidopsis thaliana (L.). The apd1 apd2 double mutant showed a significantly increased percentage of bicellular-like pollen at the mature pollen stage. Further downregulation of the APD3 and APD4 transcripts in apd1 apd2 by RNA interference (RNAi) resulted in more severe abnormal bicellular-like pollen phenotypes than in apd1 apd2, suggesting that cell division was defective in male gametogenesis. All of the four genes were expressed in multiple stages at different levels during male gametophyte development. Confocal analysis using green florescence fusion proteins (GFP) GFP-APD1 and GFP-APD2 showed that APDs are associated with intracellular membranes. Furthermore, APD2 had E2-dependent E3 ligase activity in vitro, and five APD2-interacting proteins were identified. Our results suggest that these four genes may be involved, redundantly, in regulating the PMII process during male gametogenesis.; Biochemistry & Molecular Biology; Plant Sciences; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 7; ARTICLE; 10,SI; 814-827; 54
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/191300]
专题	生命科学学院
推荐引用方式 GB/T 7714	Luo, Guo,Gu, Hongya,Liu, Jingjing,et al. Four Closely-related RING-type E3 Ligases, APD1-4, are Involved in Pollen Mitosis II Regulation in Arabidopsis[J]. journal of integrative plant biology,2012.
APA	Luo, Guo,Gu, Hongya,Liu, Jingjing,&Qu, Li-Jia.(2012).Four Closely-related RING-type E3 Ligases, APD1-4, are Involved in Pollen Mitosis II Regulation in Arabidopsis.journal of integrative plant biology.
MLA	Luo, Guo,et al."Four Closely-related RING-type E3 Ligases, APD1-4, are Involved in Pollen Mitosis II Regulation in Arabidopsis".journal of integrative plant biology (2012).