PTK7-Src Signaling at Epithelial Cell Contacts Mediates Spatial   Organization of Actomyosin and Planar Cell Polarity

doi:10.1016/j.devcel.2014.02.008

CORC > 北京大学 > 生命科学学院

	PTK7-Src Signaling at Epithelial Cell Contacts Mediates Spatial Organization of Actomyosin and Planar Cell Polarity
	Andreeva, Anna ; Lee, Jianyi ; Lohia, Madhura ; Wu, Xiaoji ; Macara, Ian G. ; Lu, Xiaowei
刊名	developmental cell
	2014
关键词	AUDITORY HAIR-CELLS MYOSIN-II ACTIVITY E-CADHERIN NEURAL-TUBE CONVERGENT EXTENSION TISSUE MORPHOGENESIS APICAL CONSTRICTION ZONULA ADHERENS SRC ADHESION
DOI	10.1016/j.devcel.2014.02.008
英文摘要	Actomyosin contractility plays a key role in tissue morphogenesis. During mammalian development, PTK7 regulates epithelial morphogenesis and planar cell polarity (PCP) through modulation of actomyosin contractility, but the underlying mechanism is unknown. Here, we show that PTK7 interacts with the tyrosine kinase Src and stimulates Src signaling along cell-cell contacts. We further identify ROCK2 as a target of junctional PTK7-Src signaling. PTK7 knockdown in cultured epithelial cells reduced the level of active Src at cell-cell contacts, resulting in delocalization of ROCK2 from cell-cell contacts and decreased junctional contractility, with a concomitant increase in actomyosin on the basal surface. Moreover, we present in vivo evidence that Src family kinase (SFK) activity is critical for PCP regulation in the auditory sensory epithelium and that PTK7-SFK signaling regulates tyrosine phosphorylation of junctional ROCK2. Together, these results delineate a PTK7-Src signaling module for spatial regulation of ROCK activity, actomyosin contractility, and epithelial PCP.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000334508800005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Cell Biology; Developmental Biology; SCI(E); PubMed; 9; ARTICLE; xl6f@virginia.edu; 1; 20-33; 29
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/189551]
专题	生命科学学院
推荐引用方式 GB/T 7714	Andreeva, Anna,Lee, Jianyi,Lohia, Madhura,et al. PTK7-Src Signaling at Epithelial Cell Contacts Mediates Spatial Organization of Actomyosin and Planar Cell Polarity[J]. developmental cell,2014.
APA	Andreeva, Anna,Lee, Jianyi,Lohia, Madhura,Wu, Xiaoji,Macara, Ian G.,&Lu, Xiaowei.(2014).PTK7-Src Signaling at Epithelial Cell Contacts Mediates Spatial Organization of Actomyosin and Planar Cell Polarity.developmental cell.
MLA	Andreeva, Anna,et al."PTK7-Src Signaling at Epithelial Cell Contacts Mediates Spatial Organization of Actomyosin and Planar Cell Polarity".developmental cell (2014).