Human Hepatocytes with Drug Metabolic Function Induced from Fibroblasts   by Lineage Reprogramming

doi:10.1016/j.stem.2014.01.008

CORC > 北京大学 > 生命科学学院

	Human Hepatocytes with Drug Metabolic Function Induced from Fibroblasts by Lineage Reprogramming
	Du, Yuanyuan ; Wang, Jinlin ; Jia, Jun ; Song, Nan ; Xiang, Chengang ; Xu, Jun ; Hou, Zhiyuan ; Su, Xiaohua ; Liu, Bei ; Jiang, Tao ; Zhao, Dongxin ; Sun, Yingli ; Shu, Jian ; Guo, Qingliang ; Yin, Ming ; Sun, Da ; Lu, Shichun ; Shi, Yan ; Deng, Hongkui
刊名	cell stem cell
	2014
关键词	CELL-DERIVED HEPATOCYTES PANCREAS PROGENITORS LIVER DIFFERENTIATION MATURATION SYSTEMS
DOI	10.1016/j.stem.2014.01.008
英文摘要	Obtaining fully functional cell types is a major challenge for drug discovery and regenerative medicine. Currently, a fundamental solution to this key problem is still lacking. Here, we show that functional human induced hepatocytes (hiHeps) can be generated from fibroblasts by overexpressing the hepatic fate conversion factors HNF1A, HNF4A, and HNF6 along with the maturation factors ATF5, PROX1, and CEBPA. hiHeps express a spectrum of phase I and II drug-metabolizing enzymes and phase III drug transporters. Importantly, the metabolic activities of CYP3A4, CYP1A2, CYP2B6, CYP2C9, and CYP2C19 are comparable between hiHeps and freshly isolated primary human hepatocytes. Transplanted hiHeps repopulate up to 30% of the livers of Tet-uPA/Rag2(-/-)/gamma c(-/-) mice and secrete more than 300 mu g/ml human ALBUMIN in vivo. Our data demonstrate that human hepatocytes with drug metabolic function can be generated by lineage reprogramming, thus providing a cell resource for pharmaceutical applications.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000332533400015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Cell & Tissue Engineering; Cell Biology; SCI(E); PubMed; 86; ARTICLE; lsc620213@aliyun.com; shiyan@pkusz.edu.cn; hongkui_deng@pku.edu.cn; 3; 394-403; 14
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/189424]
专题	生命科学学院
推荐引用方式 GB/T 7714	Du, Yuanyuan,Wang, Jinlin,Jia, Jun,et al. Human Hepatocytes with Drug Metabolic Function Induced from Fibroblasts by Lineage Reprogramming[J]. cell stem cell,2014.
APA	Du, Yuanyuan.,Wang, Jinlin.,Jia, Jun.,Song, Nan.,Xiang, Chengang.,...&Deng, Hongkui.(2014).Human Hepatocytes with Drug Metabolic Function Induced from Fibroblasts by Lineage Reprogramming.cell stem cell.
MLA	Du, Yuanyuan,et al."Human Hepatocytes with Drug Metabolic Function Induced from Fibroblasts by Lineage Reprogramming".cell stem cell (2014).