Switching Demethylation Activities between AlkB Family RNA/DNA   Demethylases through Exchange of Active-Site Residues

doi:10.1002/anie.201310050

CORC > 北京大学 > 生命科学学院

	Switching Demethylation Activities between AlkB Family RNA/DNA Demethylases through Exchange of Active-Site Residues
	Zhu, Chenxu ; Yi, Chengqi
刊名	angewandte chemie international edition
	2014
关键词	protein structures RNA recognition RNA/DNA demethylases selective inhibition substrate specificity OBESITY-ASSOCIATED FTO DNA ALKYLATION DAMAGE REPAIR ENZYME ALKB OXIDATIVE DEMETHYLATION ESCHERICHIA-COLI SUBSTRATE-SPECIFICITY CRYSTAL-STRUCTURES RNA DEMETHYLASE ADULT OBESITY MESSENGER-RNA
DOI	10.1002/anie.201310050
英文摘要	The AlkB family demethylases AlkB, FTO, and ALKBH5 recognize differentially methylated RNA/DNA substrates, which results in their distinct biological roles. Here we identify key active-site residues that contribute to their substrate specificity. Swapping such active-site residues between the demethylases leads to partially switched demethylation activities. Combined evidence from X-ray structures and enzyme kinetics suggests a role of the active-site residues in substrate recognition. Such a divergent active-site sequence may aid the design of selective inhibitors that can discriminate these homologue RNA/DNA demethylases.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000337176800013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Multidisciplinary; SCI(E); EI; PubMed; 15; ARTICLE; chengqi.yi@pku.edu.cn; 14; 3659-3662; 53
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/189405]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhu, Chenxu,Yi, Chengqi. Switching Demethylation Activities between AlkB Family RNA/DNA Demethylases through Exchange of Active-Site Residues[J]. angewandte chemie international edition,2014.
APA	Zhu, Chenxu,&Yi, Chengqi.(2014).Switching Demethylation Activities between AlkB Family RNA/DNA Demethylases through Exchange of Active-Site Residues.angewandte chemie international edition.
MLA	Zhu, Chenxu,et al."Switching Demethylation Activities between AlkB Family RNA/DNA Demethylases through Exchange of Active-Site Residues".angewandte chemie international edition (2014).