Switching Demethylation Activities between AlkB Family RNA/DNA Demethylases through Exchange of Active-Site Residues | |
Zhu, Chenxu ; Yi, Chengqi | |
刊名 | angewandte chemie international edition
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2014 | |
关键词 | protein structures RNA recognition RNA/DNA demethylases selective inhibition substrate specificity OBESITY-ASSOCIATED FTO DNA ALKYLATION DAMAGE REPAIR ENZYME ALKB OXIDATIVE DEMETHYLATION ESCHERICHIA-COLI SUBSTRATE-SPECIFICITY CRYSTAL-STRUCTURES RNA DEMETHYLASE ADULT OBESITY MESSENGER-RNA |
DOI | 10.1002/anie.201310050 |
英文摘要 | The AlkB family demethylases AlkB, FTO, and ALKBH5 recognize differentially methylated RNA/DNA substrates, which results in their distinct biological roles. Here we identify key active-site residues that contribute to their substrate specificity. Swapping such active-site residues between the demethylases leads to partially switched demethylation activities. Combined evidence from X-ray structures and enzyme kinetics suggests a role of the active-site residues in substrate recognition. Such a divergent active-site sequence may aid the design of selective inhibitors that can discriminate these homologue RNA/DNA demethylases.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000337176800013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Multidisciplinary; SCI(E); EI; PubMed; 15; ARTICLE; chengqi.yi@pku.edu.cn; 14; 3659-3662; 53 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/189405] ![]() |
专题 | 生命科学学院 |
推荐引用方式 GB/T 7714 | Zhu, Chenxu,Yi, Chengqi. Switching Demethylation Activities between AlkB Family RNA/DNA Demethylases through Exchange of Active-Site Residues[J]. angewandte chemie international edition,2014. |
APA | Zhu, Chenxu,&Yi, Chengqi.(2014).Switching Demethylation Activities between AlkB Family RNA/DNA Demethylases through Exchange of Active-Site Residues.angewandte chemie international edition. |
MLA | Zhu, Chenxu,et al."Switching Demethylation Activities between AlkB Family RNA/DNA Demethylases through Exchange of Active-Site Residues".angewandte chemie international edition (2014). |
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