Dissecting the Differentiation Process of the Preplacodal Ectoderm in   Zebrafish

doi:10.1002/DVDY.24160

CORC > 北京大学 > 生命科学学院

	Dissecting the Differentiation Process of the Preplacodal Ectoderm in Zebrafish
	Yao, Di ; Zhao, Feng ; Wu, Ying ; Wang, Jialiang ; Dong, Wei ; Zhao, Jue ; Zhu, Zuoyan ; Liu, Dong
刊名	发育动力学
	2014
关键词	Wnt Gata3 Foxi1 Fgf8 PPR OTIC PLACODE INDUCTION NEURAL INDUCTION SIGNALING CONTROLS SENSORY PLACODES XENOPUS-LAEVIS GROUND-STATE FGF BMP COMPETENCE SPECIFICATION
DOI	10.1002/DVDY.24160
英文摘要	Background: The preplacodal region (PPR) is a region of specialized ectoderm at the border of neural and nonneural ectoderm (NNE). Coordinated Bmp, Fgf, and Wnt signals are known to drive PPR development; however, the underlying mechanism is unknown. Results: We identified key components involved in PPR differentiation. The mesoderm/marginal Wnts at the early gastrula stage trigger differentiation by allowing the adjacent NNE border cells to start adopting caudal PPR fates; otherwise, the development of caudal PPR identity is hindered due to the persistent presence of gata3 mRNA. The caudal PPR fate dominates when foxi1 expression is enhanced at the late gastrula stage, and depleting Foxi1 after 6 hours postfertilization (hpf) reduces the otic-epibranchial placodal domain. When the Gata3 level is manipulated at the fertilized egg stage or near 6 hpf, the lens is always affected. In establishing PPR polarity, both Gata3 and Foxi1 inhibit Bmp signaling, whereas Foxi1 inhibits, but Gata3 enhances, Fgf sensitivity of the PPR cells. Conclusions: Our study reveals that in zebrafish, (1) the PPR at the shield stage may enter a developmental state when the PPR cells preferentially adopt a particular placodal fate and (2) a network of genetically linked factors, including Wnt/beta-catenin, Fgfr, Bmp, Gata3, and Foxi1, direct the process of PPR differentiation. (C) 2014 Wiley Periodicals, Inc.; Anatomy & Morphology; Developmental Biology; SCI(E); PubMed; 2; ARTICLE; doliu@pku.edu.cn; 10; 1338-1351; 243
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/188881]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yao, Di,Zhao, Feng,Wu, Ying,et al. Dissecting the Differentiation Process of the Preplacodal Ectoderm in Zebrafish[J]. 发育动力学,2014.
APA	Yao, Di.,Zhao, Feng.,Wu, Ying.,Wang, Jialiang.,Dong, Wei.,...&Liu, Dong.(2014).Dissecting the Differentiation Process of the Preplacodal Ectoderm in Zebrafish.发育动力学.
MLA	Yao, Di,et al."Dissecting the Differentiation Process of the Preplacodal Ectoderm in Zebrafish".发育动力学 (2014).