Multiple mechanisms determine the order of APC/C substrate degradation   in mitosis

doi:10.1083/jcb.201402041

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	Multiple mechanisms determine the order of APC/C substrate degradation in mitosis
	Lu, Dan ; Hsiao, Jennifer Y. ; Davey, Norman E. ; Van Voorhis, Vanessa A. ; Foster, Scott A. ; Tang, Chao ; Morgan, David O.
刊名	journal of cell biology
	2014
关键词	SPINDLE-ASSEMBLY CHECKPOINT ANAPHASE-PROMOTING COMPLEX SISTER-CHROMATID SEPARATION SACCHAROMYCES-CEREVISIAE BUDDING YEAST CYCLIN-A CELL-CYCLE MITOTIC EXIT DEPENDENT PROTEOLYSIS PROTEIN-KINASE
DOI	10.1083/jcb.201402041
英文摘要	The ubiquitin protein ligase anaphase-promoting complex or cyclosome (APC/C) controls mitosis by promoting ordered degradation of securin, cyclins, and other proteins. The mechanisms underlying the timing of APC/C substrate degradation are poorly understood. We explored these mechanisms using quantitative fluorescence microscopy of GFP-tagged APC/C-Cdc20 substrates in living budding yeast cells. Degradation of the S cyclin, Clb5, begins early in mitosis, followed 6 min later by the degradation of securin and Dbf4. Anaphase begins when less than half of securin is degraded. The spindle assembly checkpoint delays the onset of Clb5 degradation but does not influence securin degradation. Early Clb5 degradation depends on its interaction with the Cdk1-Cks1 complex and the presence of a Cdc20-binding "ABBA motif" in its N-terminal region. The degradation of securin and Dbf4 is delayed by Cdk1-dependent phosphorylation near their Cdc20-binding sites. Thus, a remarkably diverse array of mechanisms generates robust ordering of APC/C-Cdc20 substrate destruction.; Cell Biology; SCI(E); PubMed; 6; ARTICLE; tangc@pku.edu.cn; david.morgan@ucsf.edu; 1; 23-39; 207
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/188784]
专题	生命科学学院
推荐引用方式 GB/T 7714	Lu, Dan,Hsiao, Jennifer Y.,Davey, Norman E.,et al. Multiple mechanisms determine the order of APC/C substrate degradation in mitosis[J]. journal of cell biology,2014.
APA	Lu, Dan.,Hsiao, Jennifer Y..,Davey, Norman E..,Van Voorhis, Vanessa A..,Foster, Scott A..,...&Morgan, David O..(2014).Multiple mechanisms determine the order of APC/C substrate degradation in mitosis.journal of cell biology.
MLA	Lu, Dan,et al."Multiple mechanisms determine the order of APC/C substrate degradation in mitosis".journal of cell biology (2014).