Apigenin attenuates heart injury in lipopolysaccharide-induced endotoxemic model by suppressing sphingosine kinase 1/sphingosine 1-phosphate signaling pathway

doi:10.1016/j.cbi.2014.12.021

CORC > 北京大学 > 生命科学学院

	Apigenin attenuates heart injury in lipopolysaccharide-induced endotoxemic model by suppressing sphingosine kinase 1/sphingosine 1-phosphate signaling pathway
	Zhang Tianzhu ; Yan Tianhua ; Du Juan ; Wang Shumin ; Yang Huilin
刊名	化学与生物的相互作用
	2015
关键词	Apigenin,Endotoxemic heart,SphK1/S1P signaling pathway
DOI	10.1016/j.cbi.2014.12.021
英文摘要	Sepsis is a cluster of heterogeneous syndromes associated with progressive endotoxemic developments, ultimately leading to damage of multiple organs, including the heart. This study is to investigate the effects of apigenin on heart injury in lipopolysaccharide-induced endotoxemic rat model. Normal Wistar rats were randomly divided into four groups: control group, LPS group (15mg/kg), LPS plus apigenin groups with different apigenin doses (50mg/kg, 100mg/kg). Serum levels of creatine kinase (CK), lactate dehydrogenase (LDH), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β) were measured after the rats were sacrificed. SphK1/S1P signaling pathway proteins, cleaved caspase-3, cleaved caspase-9, Bax and Bcl-2 in heart were measured by Western blot. In vitro, we evaluated the protective effect of apigenin on rat embryonic heart-derived myogenic cell line H9c2 induced by LPS. Apigenin decreased serum levels of CK-MB, LDH, TNF-α, IL-6, IL-1β. SphK1/S1P signaling pathway proteins, cleaved caspase-3, cleaved caspase-9, Bax in heart were found inhibited and Bcl-2 increased in the apigenin groups in vivo. In addition, apigenin inhibited intracellular calcium, the MAPK pathway and SphK1/S1P signaling pathway in vitro. Apigenin exerts pronounced cardioprotection in rats subjected to LPS likely through suppressing myocardial apoptosis and inflammation by inhibiting the SphK1/S1P signaling pathway.Copyright ? 2015 Elsevier Ireland Ltd. All rights reserved.; SCI(E); PubMed; 0; ARTICLE; yantianhuabest@126.com; wangshuminch@126.com; 46-55; 233
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/188633]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang Tianzhu,Yan Tianhua,Du Juan,et al. Apigenin attenuates heart injury in lipopolysaccharide-induced endotoxemic model by suppressing sphingosine kinase 1/sphingosine 1-phosphate signaling pathway[J]. 化学与生物的相互作用,2015.
APA	Zhang Tianzhu,Yan Tianhua,Du Juan,Wang Shumin,&Yang Huilin.(2015).Apigenin attenuates heart injury in lipopolysaccharide-induced endotoxemic model by suppressing sphingosine kinase 1/sphingosine 1-phosphate signaling pathway.化学与生物的相互作用.
MLA	Zhang Tianzhu,et al."Apigenin attenuates heart injury in lipopolysaccharide-induced endotoxemic model by suppressing sphingosine kinase 1/sphingosine 1-phosphate signaling pathway".化学与生物的相互作用 (2015).