An AP Endonuclease Functions in Active DNA Dimethylation and Gene   Imprinting in Arabidopsis

doi:10.1371/journal.pgen.1004905

CORC > 北京大学 > 生命科学学院

	An AP Endonuclease Functions in Active DNA Dimethylation and Gene Imprinting in Arabidopsis
	Li, Yan ; Cordoba-Canero, Dolores ; Qian, Weiqiang ; Zhu, Xiaohong ; Tang, Kai ; Zhang, Huiming ; Ariza, Rafael R. ; Roldan-Arjona, Teresa ; Zhu, Jian-Kang
刊名	plos genetics
	2015
关键词	BASE EXCISION-REPAIR SEED DEVELOPMENT POLYCOMB GENE DEMETHYLATION METHYLATION GLYCOSYLASE DEMETER PLANTS PROTEIN ENDOSPERM
DOI	10.1371/journal.pgen.1004905
英文摘要	Active DNA demethylation in plants occurs through base excision repair, beginning with removal of methylated cytosine by the ROS1/DME subfamily of 5-methylcytosine DNA glycosylases. Active DNA demethylation in animals requires the DNA glycosylase TDG or MBD4, which functions after oxidation or deamination of 5-methylcytosine, respectively. However, little is known about the steps following DNA glycosylase action in the active DNA demethylation pathways in plants and animals. We show here that the Arabidopsis APE1L protein has apurinic/apyrimidinic endonuclease activities and functions downstream of ROS1 and DME. APE1L and ROS1 interact in vitro and co-localize in vivo. Whole genome bisulfite sequencing of ape1l mutant plants revealed widespread alterations in DNA methylation. We show that the ape1l/zdp double mutant displays embryonic lethality. Notably, the ape1l(+/-) zdp(-/-) mutant shows a maternal-effect lethality phenotype. APE1L and the DNA phosphatase ZDP are required for FWA and MEA gene imprinting in the endosperm and are important for seed development. Thus, APE1L is a new component of the active DNA demethylation pathway and, together with ZDP, regulates gene imprinting in Arabidopsis.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000349314600025&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Genetics & Heredity; SCI(E); PubMed; 6; ARTICLE; ge2roarm@uco.es; jkzhu@purdue.edu; 1; e1004905; 11
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/188619]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, Yan,Cordoba-Canero, Dolores,Qian, Weiqiang,et al. An AP Endonuclease Functions in Active DNA Dimethylation and Gene Imprinting in Arabidopsis[J]. plos genetics,2015.
APA	Li, Yan.,Cordoba-Canero, Dolores.,Qian, Weiqiang.,Zhu, Xiaohong.,Tang, Kai.,...&Zhu, Jian-Kang.(2015).An AP Endonuclease Functions in Active DNA Dimethylation and Gene Imprinting in Arabidopsis.plos genetics.
MLA	Li, Yan,et al."An AP Endonuclease Functions in Active DNA Dimethylation and Gene Imprinting in Arabidopsis".plos genetics (2015).