小白鼠心肌核DNA片段中的基因表达的分子开关的研究; The Study on the Molecular Switch of Gene Expression of the Mouse Heart Nuclear DNA Fragments | |
袁明秀 ; 侯仲刚 ; 陈志俊 ; 张志宏 ; 邓爱平 | |
刊名 | 高技术通讯
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2001 | |
关键词 | 基因表达分子开关 正负反馈调控机制 基因表达与调控 AFM |
DOI | 10.3321/j.issn:1002-0470.2001.01.002 |
英文摘要 | 原位染色发现,LDH(1-5)…nNAD+(LDH:乳酸脱氢酶,NAD+:氢化型辅酶I)可以 进入核孔,可能与自己编码基因特异结合。体外表达 实验中,稀释心肌DNA片段,促进LDH/DNA*表达LDH(1-5), LDH (1-5)表达量与LDH/DNA*中可 解离的LDH(1-5)量呈正相关性。同位素14C标记Leu法测定加入LDH(1-5) …nNAD+可以抑制LD H/DNA*体外表达,LDH(1-5)表达抑制量与加入LDH(1-5)…nNAD+量呈正相 关性。AFM直接观察 到活性基因两头的调控序列可能结合自己编码的蛋白等活性因子。基因表达与调控的分子开 关主要是自己编码的蛋白等活性因子。展示了未来运用AFM和同位素标记法相结合研 究生物学反应的前景。; It is observed by in situ stain that LDH(1-5)…nNAD+ can probabl y enter the nucleopore and bind specifically with the gene that encodes itself. During the i n vitro expression, the dilution of heart nuclear DNA fragments could enhance th e expression activity of LDH/DNA*, the amount of expressed LDH(1-5) is in propo rtion to the amount of dissociable LDH(1-5) on the LDH/DNA*. With the int egrati on of the 14 C-Leu to the protein, it is also observed that the additio n of LDH (1-5)…nNAD+ may suppress the in vitro expression activity of LDH/DNA* and the decreased amount of expressed LDH(1-5) is in proportion to the amount of a dded L DH(1-5)…nNAD+. AFM observation shows that the regulation sequence at t he two e nds of the active genes may bind with such active factors as the proteins encode d by the genes themselves.; 中国科学引文数据库(CSCD); 0; 1; 7-13; 11 |
语种 | 中文 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/179999] ![]() |
专题 | 生命科学学院 |
推荐引用方式 GB/T 7714 | 袁明秀,侯仲刚,陈志俊,等. 小白鼠心肌核DNA片段中的基因表达的分子开关的研究, The Study on the Molecular Switch of Gene Expression of the Mouse Heart Nuclear DNA Fragments[J]. 高技术通讯,2001. |
APA | 袁明秀,侯仲刚,陈志俊,张志宏,&邓爱平.(2001).小白鼠心肌核DNA片段中的基因表达的分子开关的研究.高技术通讯. |
MLA | 袁明秀,et al."小白鼠心肌核DNA片段中的基因表达的分子开关的研究".高技术通讯 (2001). |
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