抗真菌肽LP-1的分离纯化及特性分析

CORC > 北京大学 > 生命科学学院

	抗真菌肽LP-1的分离纯化及特性分析; Purification and characterization of antifungal peptide LP-1
	刘颖 ; 徐庆 ; 陈章良
刊名	微生物学报
	1999
关键词	枯草芽孢杆菌TG-26 抗菌肽抑菌谱环肽
英文摘要	拮抗菌枯草芽孢杆菌（Ｂａｃｉｌｌｕｓｓｕｂｔｉｌｉｓ）ＴＧ２６分泌产生的小肽经两次盐酸沉淀、丙酮分级沉淀和Ｈｉｐｏｒｅ反相柱两次纯化，分离得到一种新的抗真菌的小肽，命名为ＬＰ１。经ＭＡＬＤＩＴＯＦ质谱鉴定，分子量为１０５７－３，等电聚焦测得其ｐＩ为４－７５。ＬＰ１对温度有较高的稳定性，１００ ℃保温３０ｍｉｎ，仍能保持７５％的活性。抑菌谱表明，该抗菌肽对瓜果腐霉（Ｐｙｔｈｉｕｍａｐｈａｎｉｄｅｒｍａｔｕｍ）、玉蜀黍赤霉病菌（Ｇｉｂｂｅｒｅｌｌａｚｅａｅ）、长柄链格孢（Ａｌｔｅｒｎａｒｉａｌｏｎｇｉｐｅ）和番茄蔫萎座镰孢霉（Ｆｕｓａｒｉｕｍ...; An antifungal peptide LP-1 from Bacillus subtilis TG26 strain was purified by acid precipitation, acetone precipitation and Hi-pore reversed phase column chromatography. The molecular weight of LP-1 is 1057.3 D as determined by MALDI-TOF mass spectrometry, and its pI is 4.75 by PAG-IEF. It was also found to be thermostable. Its antifungal spectrum showed that LP-1 has strong inhibitory activity against many plant pathogenic fungi, such as Pythium aphanidermatum, Gibberella zeae, Alternaria longipe, Fusarium oxysporum f. lycopersici, etc.. The abnormal hyphal growth of Trichoderma viride caused by LP-1 such as swollen tips, twisted, short growth and cytoplasm condensation was also observed. Both ninhydrin reaction and peptide sequencing suggested that LP-1 is a cyclic peptide.; PubMed; 中文核心期刊要目总览(PKU); 中国科学引文数据库(CSCD); 0; 05; 441-447; 39
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/170432]
专题	生命科学学院
推荐引用方式 GB/T 7714	刘颖,徐庆,陈章良. 抗真菌肽LP-1的分离纯化及特性分析, Purification and characterization of antifungal peptide LP-1[J]. 微生物学报,1999.
APA	刘颖,徐庆,&陈章良.(1999).抗真菌肽LP-1的分离纯化及特性分析.微生物学报.
MLA	刘颖,et al."抗真菌肽LP-1的分离纯化及特性分析".微生物学报 (1999).