In vitro bioactivity and biocompatibility evaluation of bulk   nanostructured titanium in osteoblast-like cells by quantitative   proteomic analysis

doi:10.1039/c3tb00266g

CORC > 北京大学 > 生命科学学院

	In vitro bioactivity and biocompatibility evaluation of bulk nanostructured titanium in osteoblast-like cells by quantitative proteomic analysis
	Zhao, Minzhi ; Wang, Qingsong ; Lai, Wenjia ; Zhao, Xuyang ; Shen, Hongyan ; Nie, Feilong ; Zheng, Yufeng ; Wei, Shicheng ; Ji, Jianguo
刊名	journal of materials chemistry b
	2013
关键词	SEVERE PLASTIC-DEFORMATION MECHANICAL-PROPERTIES DEUBIQUITINATING ENZYMES SUPEROXIDE-DISMUTASE UBIQUITIN DIFFERENTIATION PROTEASOME PROTEINS ALLOYS MASS
DOI	10.1039/c3tb00266g
英文摘要	Nanostructured titanium prepared by the equal-channel angular pressing route (ECAPed Ti) has shown great promise as an implant material over conventional pure titanium. The aim of this report is to investigate its biological properties, surface performance, and comprehensive biological effects at a molecular level when in contact with cells. Protein expression changes of human osteoblast-like MG-63 in response to polished ECAPed Ti had been profiled by employing stable isotope labelling with amino acids in cell culture (SILAC), using cpTi as control after the same polishing process. It was found that ubiquitin proteasome related processes were predominantly enriched in the over-expressed proteins. Superoxide dismutase 2 (SOD2) was apparently up-regulated on the ECAPed Ti surface, which could have contributed to the increase in SOD activity and the decrease in the reactive oxygen species (ROS) level. These expression changes have relationships with protein degradation, bone formation and resistance to oxidative injury, and they suggest that ECAPed Ti has the potential to further promote osteoblast differentiation. On the other hand, the down-regulated proteins exhibited resistance to platelet adhesion on the ECAPed Ti surface. This study reveals the differential expression of proteins in human osteoblasts induced by nanostructured titanium substrates for the first time.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000316120800007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Materials Science, Biomaterials; SCI(E); EI; 6; ARTICLE; 14; 1926-1938; 1
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/164128]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhao, Minzhi,Wang, Qingsong,Lai, Wenjia,et al. In vitro bioactivity and biocompatibility evaluation of bulk nanostructured titanium in osteoblast-like cells by quantitative proteomic analysis[J]. journal of materials chemistry b,2013.
APA	Zhao, Minzhi.,Wang, Qingsong.,Lai, Wenjia.,Zhao, Xuyang.,Shen, Hongyan.,...&Ji, Jianguo.(2013).In vitro bioactivity and biocompatibility evaluation of bulk nanostructured titanium in osteoblast-like cells by quantitative proteomic analysis.journal of materials chemistry b.
MLA	Zhao, Minzhi,et al."In vitro bioactivity and biocompatibility evaluation of bulk nanostructured titanium in osteoblast-like cells by quantitative proteomic analysis".journal of materials chemistry b (2013).