Identification of a novel nuclear-localized adenylate kinase from   Drosophila melanogaster

doi:10.1134/S0006297908010057

CORC > 北京大学 > 生命科学学院

	Identification of a novel nuclear-localized adenylate kinase from Drosophila melanogaster
	Meng, Geng ; Zhai, Ruitong ; Liu, Bin ; Zheng, Xiaofeng
刊名	biochemistry moscow
	2008
关键词	cloning protein expression adenylate kinase activity nuclear localization CDNA CLONING PROTEIN EXPRESSION TOOLS
DOI	10.1134/S0006297908010057
英文摘要	As a step to further understand the role of adenylate kinase (AK) in the energy metabolism network, we identified, purified, and characterized a previously undescribed adenylate kinase in Drosophila melanogaster. The cDNA encodes a 175-amino acid protein, which shows 47.85% identity in 163 amino acids to human AK6. The recombinant protein was successfully expressed in Escherichia coli BL21(DE3) strain. Characterization of this protein by enzyme activity assay showed adenylate kinase activity. AMP and CMP were the preferred substrates, and UMP can also be phosphorylated to some extent, with ATP as the best phosphate donor. Subcellular localization study showed a predominantly nuclear localization. Therefore, based on the substrate specificity, the specific nuclear localization in the cell, and the sequence similarity with human AK6, we named this novel adenylate kinase identified from the fly DAK6.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000253311400005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; SCI(E); EI; 6; ARTICLE; 1; 38-43; 73
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162708]
专题	生命科学学院
推荐引用方式 GB/T 7714	Meng, Geng,Zhai, Ruitong,Liu, Bin,et al. Identification of a novel nuclear-localized adenylate kinase from Drosophila melanogaster[J]. biochemistry moscow,2008.
APA	Meng, Geng,Zhai, Ruitong,Liu, Bin,&Zheng, Xiaofeng.(2008).Identification of a novel nuclear-localized adenylate kinase from Drosophila melanogaster.biochemistry moscow.
MLA	Meng, Geng,et al."Identification of a novel nuclear-localized adenylate kinase from Drosophila melanogaster".biochemistry moscow (2008).