Cullin Mediates Degradation of RhoA through Evolutionarily Conserved BTB   Adaptors to Control Actin Cytoskeleton Structure and Cell Movement

doi:10.1016/j.molcel.2009.09.004

CORC > 北京大学 > 生命科学学院

	Cullin Mediates Degradation of RhoA through Evolutionarily Conserved BTB Adaptors to Control Actin Cytoskeleton Structure and Cell Movement
	Chen, Yuezhou ; Yang, Zhenxiao ; Meng, Min ; Zhao, Yue ; Dong, Na ; Yan, Hongming ; Liu, Liping ; Ding, Mingxiao ; Peng, H. Benjamin ; Shao, Feng
刊名	分子细胞
	2009
关键词	CUL3-BASED E3 LIGASE CONVERGENT EXTENSION MOVEMENTS UBIQUITIN-LIGASE EMBRYONIC-DEVELOPMENT XENOPUS GASTRULATION DOMAIN PROTEINS CYCLIN-E GTPASES PATHWAY BINDING
DOI	10.1016/j.molcel.2009.09.004
英文摘要	Cul3, a Cullin family scaffold protein, is thought to mediate the assembly of a large number of SCF (Skp1-Cullin1-F-box protein)-like ubiquitin ligase complexes through BTB domain substrate-recruiting adaptors. Cul3 controls early embryonic development in several genetic models through mechanisms not understood. Very few functional substrate/adaptor pairs for CUB ubiquitin ligases have been identified. Here, we show that Cul3 knockdown in human cells results in abnormal actin stress fibers and distorted cell morphology, owing to impaired ubiquitination and degradation of small GTPase RhoA. We identify a family of RhoA-binding BTB domain adaptors conserved from insects to mammals, designated BACURDs. BACURDs form ubiquitin ligase complexes, which selectively ubiquitinate RhoA, with Cul3. Dysfunction of the Cul3/BACURD complex decreases cell migration potential and impairs RhoA-mediated convergent extension movements during Xenopus gastrulation. Our studies reveal a previously unknown mechanism for controlling RhoA degradation and regulating RhoA function in various biological contexts, which involves a Cul3/BACILIRD ubiquitin ligase complex.; Biochemistry & Molecular Biology; Cell Biology; SCI(E); PubMed; 71; ARTICLE; 6; 841-855; 35
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162564]
专题	生命科学学院
推荐引用方式 GB/T 7714	Chen, Yuezhou,Yang, Zhenxiao,Meng, Min,et al. Cullin Mediates Degradation of RhoA through Evolutionarily Conserved BTB Adaptors to Control Actin Cytoskeleton Structure and Cell Movement[J]. 分子细胞,2009.
APA	Chen, Yuezhou.,Yang, Zhenxiao.,Meng, Min.,Zhao, Yue.,Dong, Na.,...&Shao, Feng.(2009).Cullin Mediates Degradation of RhoA through Evolutionarily Conserved BTB Adaptors to Control Actin Cytoskeleton Structure and Cell Movement.分子细胞.
MLA	Chen, Yuezhou,et al."Cullin Mediates Degradation of RhoA through Evolutionarily Conserved BTB Adaptors to Control Actin Cytoskeleton Structure and Cell Movement".分子细胞 (2009).