Silkworm Coatomers and Their Role in Tube Expansion of Posterior   Silkgland

doi:10.1371/journal.pone.0013252

CORC > 北京大学 > 生命科学学院

	Silkworm Coatomers and Their Role in Tube Expansion of Posterior Silkgland
	Wang, Qiao ; Shen, Birong ; Zheng, Pengli ; Feng, Hui ; Chen, Liang ; Zhang, Jing ; Zhang, Chuanxi ; Zhang, Guozheng ; Teng, Junlin ; Chen, Jianguo
刊名	plos one
	2010
关键词	BOMBYX-MORI TARGETED DISRUPTION INTRACELLULAR-TRANSPORT ENDOPLASMIC-RETICULUM MEMBRANE-TRANSPORT SEQUENCE ALIGNMENT PROTEIN-TRANSPORT GOLGI TRANSPORT COAT PROTEINS GAMMA-COP
DOI	10.1371/journal.pone.0013252
英文摘要	Background: Coat protein complex I (COPI) vesicles, coated by seven coatomer subunits, are mainly responsible for Golgito- ER transport. Silkworm posterior silkgland (PSG), a highly differentiated secretory tissue, secretes fibroin for silk production, but many physiological processes in the PSG cells await further investigation. Methodology/Principal Findings: Here, to investigate the role of silkworm COPI, we cloned six silkworm COPI subunits (alpha,beta,beta', delta, epsilon, and zeta-COP), determined their peak expression in day 2 in fifth-instar PSG, and visualized the localization of COPI, as a coat complex, with cis-Golgi. By dsRNA injection into silkworm larvae, we suppressed the expression of alpha-, beta'- and gamma-COP, and demonstrated that COPI subunits were required for PSG tube expansion. Knockdown of alpha-COP disrupted the integrity of Golgi apparatus and led to a narrower glandular lumen of the PSG, suggesting that silkworm COPI is essential for PSG tube expansion. Conclusions/Significance: The initial characterization reveals the essential roles of silkworm COPI in PSG. Although silkworm COPI resembles the previously characterized coatomers in other organisms, some surprising findings require further investigation. Therefore, our results suggest the silkworm as a model for studying intracellular transport, and would facilitate the establishment of silkworm PSG as an efficient bioreactor.; Multidisciplinary Sciences; SCI(E); PubMed; 8; ARTICLE; 10; e13252; 5
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162547]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, Qiao,Shen, Birong,Zheng, Pengli,et al. Silkworm Coatomers and Their Role in Tube Expansion of Posterior Silkgland[J]. plos one,2010.
APA	Wang, Qiao.,Shen, Birong.,Zheng, Pengli.,Feng, Hui.,Chen, Liang.,...&Chen, Jianguo.(2010).Silkworm Coatomers and Their Role in Tube Expansion of Posterior Silkgland.plos one.
MLA	Wang, Qiao,et al."Silkworm Coatomers and Their Role in Tube Expansion of Posterior Silkgland".plos one (2010).