A High-Throughput Screening System for Arabidopsis Transcription Factors   and Its Application to Med25-Dependent Transcriptional Regulation

doi:10.1093/mp/ssr002

CORC > 北京大学 > 生命科学学院

	A High-Throughput Screening System for Arabidopsis Transcription Factors and Its Application to Med25-Dependent Transcriptional Regulation
	Ou, Bin ; Yin, Kang-Quan ; Liu, Sai-Nan ; Yang, Yan ; Gu, Tren ; Hui, Jennifer Man Wing ; Zhang, Li ; Miao, Jin ; Kondou, Youichi ; Matsui, Minami ; Gu, Hong-Ya ; Qu, Li-Jia
刊名	分子植物
	2011
关键词	Transcription factors Arabidopsis yeast-one-hybrid yeast-two-hybrid Med25 PROTEIN-PROTEIN INTERACTIONS PLANT DEFENSIN GENE RNA-POLYMERASE-II ONE-HYBRID SYSTEM SALICYLIC-ACID MEDIATOR COMPLEX FLOWERING-TIME PR-1 GENE JASMONATE ACTIVATION
DOI	10.1093/mp/ssr002
英文摘要	The activities of transcription factors (TFs) require interactions with specific DNA sequences and other regulatory proteins. To detect such interactions in Arabidopsis, we developed a high-throughput screening system with a Gateway-compatible Gal4-AD-TF library of 1589 Arabidopsis TFs, which can be easily screened by mating-based yeast-one-hybrid (Y1H) and yeast-two-hybrid (Y2H) methods. The efficiency of the system was validated by examining two well-characterized TF-DNA and TF-protein interactions: the CHE-CCA1 promoter interaction by Y1H and NPR1-TGAs interactions by Y2H. We used this system to identify eight TFs that interact with a Mediator subunit, Med25, a key regulator in JA signaling. We identified five TFs that interacted with the GCC-box cis-element in the promoter of PDF1.2, a downstream gene of Med25. We found that three of these TFs, all from the AP2-EREBP family, interact directly both with Med25 and the GCC-box of PDF1.2, suggesting that Med25 regulates PDF1.2 expression through these three TFs. These results demonstrate that this high-throughput Y1H/Y2H screening system is an efficient tool for studying transcriptional regulation networks in Arabidopsis. This system will be available for other Arabidopsis researchers, and thus it provides a vital resource for the Arabidopsis community.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000290813600015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Plant Sciences; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 60; ARTICLE; 3; 546-555; 4
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162541]
专题	生命科学学院
推荐引用方式 GB/T 7714	Ou, Bin,Yin, Kang-Quan,Liu, Sai-Nan,et al. A High-Throughput Screening System for Arabidopsis Transcription Factors and Its Application to Med25-Dependent Transcriptional Regulation[J]. 分子植物,2011.
APA	Ou, Bin.,Yin, Kang-Quan.,Liu, Sai-Nan.,Yang, Yan.,Gu, Tren.,...&Qu, Li-Jia.(2011).A High-Throughput Screening System for Arabidopsis Transcription Factors and Its Application to Med25-Dependent Transcriptional Regulation.分子植物.
MLA	Ou, Bin,et al."A High-Throughput Screening System for Arabidopsis Transcription Factors and Its Application to Med25-Dependent Transcriptional Regulation".分子植物 (2011).