Preliminary X-ray crystallographic studies of the catalytic subunit of   Escherichia coli AHAS II with its cofactors

doi:10.1107/S1744309111008839

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	Preliminary X-ray crystallographic studies of the catalytic subunit of Escherichia coli AHAS II with its cofactors
	Niu, Xuhui ; Liu, Xiang ; Zhou, Yanfei ; Niu, Congwei ; Xi, Zhen ; Su, Xiao-Dong
刊名	acta crystallographica section f structural biology and crystallization communications
	2011
关键词	ACETOHYDROXYACID SYNTHASE INHIBITION BINDING
DOI	10.1107/S1744309111008839
英文摘要	Acetohydroxyacid synthase (AHAS) is the first common enzyme in the branched-chain amino-acid biosynthesis pathway and is the target of several classes of commercial herbicides. In this study, the Escherichia coli ilvG gene that encodes the catalytic subunit of AHAS II was cloned into the pET28a vector and expressed in soluble form at high levels in E. coli strain BL21 (DE3) cells. The protein was purified using Ni2+-chelating chromatography followed by size-exclusion chromatography. The catalytic subunit of E. coli AHAS II was cocrystallized with its cofactors Mg2+, FAD and ThDP using the sitting-drop vapour-diffusion method and the crystals diffracted to 2.80 angstrom resolution.; Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography; SCI(E); PubMed; 5; ARTICLE; Pt 6; 659-661; 67
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162527]
专题	生命科学学院
推荐引用方式 GB/T 7714	Niu, Xuhui,Liu, Xiang,Zhou, Yanfei,et al. Preliminary X-ray crystallographic studies of the catalytic subunit of Escherichia coli AHAS II with its cofactors[J]. acta crystallographica section f structural biology and crystallization communications,2011.
APA	Niu, Xuhui,Liu, Xiang,Zhou, Yanfei,Niu, Congwei,Xi, Zhen,&Su, Xiao-Dong.(2011).Preliminary X-ray crystallographic studies of the catalytic subunit of Escherichia coli AHAS II with its cofactors.acta crystallographica section f structural biology and crystallization communications.
MLA	Niu, Xuhui,et al."Preliminary X-ray crystallographic studies of the catalytic subunit of Escherichia coli AHAS II with its cofactors".acta crystallographica section f structural biology and crystallization communications (2011).