Characterization of an ethylene-inducible, calcium-dependent nuclease   that is differentially expressed in cucumber flower development

doi:10.1111/j.1469-8137.2011.03825.x

CORC > 北京大学 > 生命科学学院

	Characterization of an ethylene-inducible, calcium-dependent nuclease that is differentially expressed in cucumber flower development
	Gu, Hai-Tao ; Wang, Dong-Hui ; Li, Xia ; He, Chao-Xing ; Xu, Zhi-Hong ; Bai, Shu-Nong
刊名	new phytologist
	2011
关键词	CsCaN cucumber (Cucumis sativus) DNA damage ethylene induction unisexual flowers PROGRAMMED CELL-DEATH TRIGGER DNA FRAGMENTATION SEX DETERMINATION SATIVUS L STAPHYLOCOCCAL NUCLEASE ACTIVATED DNASE ENDONUCLEASE-G SYNTHASE GENE FEMALE FLOWER PLANTS
DOI	10.1111/j.1469-8137.2011.03825.x
英文摘要	Production of unisexual flowers is an important mechanism that promotes cross-pollination in angiosperms. We previously identified primordial anther-specific DNA damage and organ-specific ethylene perception responsible for the arrest of stamen development in female flowers, but little is known about how the two processes are linked. To identify potential links between the two processes, we performed suppression subtractive hybridization (SSH) on cucumber (Cucumis sativus L.) stamens of male and female flowers at stage 6, with stamens at stage 5 of bisexual flowers as a control. Among the differentially expressed genes, we identified an expressed sequence tag (EST) encoding a cucumber homolog to an Arabidopsis calcium-dependent nuclease (CAN), designated CsCaN. Full-length CsCaN cDNA and the respective genomic DNA sequence were cloned and characterized. The CsCaN protein exhibited calcium-dependent nuclease activity. CsCaN showed ubiquitous expression; however, increased gene expression was detected in the stamens of stage 6 female flowers compared with male flowers. As expected, CsCaN expression was ethylene inducible. It was of great interest that CsCaN was post-translationally modified. This study demonstrated that CsCaN is a novel cucumber nuclease gene, whose DNase activity is regulated at multiple levels, and which could be involved in the primordial anther-specific DNA damage of developing female cucumber flowers.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000296850800007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Plant Sciences; SCI(E); PubMed; 10; ARTICLE; 3; 590-600; 192
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162524]
专题	生命科学学院
推荐引用方式 GB/T 7714	Gu, Hai-Tao,Wang, Dong-Hui,Li, Xia,et al. Characterization of an ethylene-inducible, calcium-dependent nuclease that is differentially expressed in cucumber flower development[J]. new phytologist,2011.
APA	Gu, Hai-Tao,Wang, Dong-Hui,Li, Xia,He, Chao-Xing,Xu, Zhi-Hong,&Bai, Shu-Nong.(2011).Characterization of an ethylene-inducible, calcium-dependent nuclease that is differentially expressed in cucumber flower development.new phytologist.
MLA	Gu, Hai-Tao,et al."Characterization of an ethylene-inducible, calcium-dependent nuclease that is differentially expressed in cucumber flower development".new phytologist (2011).