A Global Identification and Analysis of Small Nucleolar RNAs and   Possible Intermediate-Sized Non-Coding RNAs in Oryza sativa

doi:10.1093/mp/sss087

CORC > 北京大学 > 生命科学学院

	A Global Identification and Analysis of Small Nucleolar RNAs and Possible Intermediate-Sized Non-Coding RNAs in Oryza sativa
	Liu, Ting-Ting ; Zhu, Danmeng ; Chen, Wei ; Deng, Wei ; He, Hang ; He, Guangming ; Bai, Baoyan ; Qi, Yijun ; Chen, Runsheng ; Deng, Xing Wang
刊名	分子植物
	2013
关键词	intermediate-size non-coding RNA small nucleolar RNA rice GENE-EXPRESSION RICE GENOME EVOLUTION SNORNAS TRANSCRIPTION FAMILIES SEQUENCE ORGANIZATION DUPLICATION ANNOTATION
DOI	10.1093/mp/sss087
英文摘要	Accumulating evidence suggests that non-coding RNAs (ncRNAs) are both widespread and functionally important in many eukaryotic organisms. In this study, we employed a special size fractionation and cDNA library construction method followed by 454 deep sequencing to systematically profile rice intermediate-size ncRNAs. Our analysis resulted in the identification of 1349 ncRNAs in total, including 754 novel ncRNAs of an unknown functional category. Chromosome distribution of all identified ncRNAs showed no strand bias, and displayed a pattern similar to that observed in protein-coding genes with few chromosome dependencies. More than half of the ncRNAs were centered around the plus-strand of the 5 and 3 termini of the coding regions. The majority of the novel ncRNAs were rice specific, while 78% of the small nucleolar RNAs (snoRNAs) were conserved. Tandem duplication drove the expansion of over half of the snoRNA gene families. Furthermore, 90% of the snoRNA candidates were shown to produce small RNAs between 2030 nt, 80% of which were associated with ARGONAUT proteins generally, and AGO1b in particular. Overall, our findings provide a comprehensive view of an intermediate-size non-coding transcriptome in a monocot species, which will serve as a useful platform for an in-depth analysis of ncRNA functions.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000319476300021&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Plant Sciences; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 10; ARTICLE; 3; 830-846; 6
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162507]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu, Ting-Ting,Zhu, Danmeng,Chen, Wei,et al. A Global Identification and Analysis of Small Nucleolar RNAs and Possible Intermediate-Sized Non-Coding RNAs in Oryza sativa[J]. 分子植物,2013.
APA	Liu, Ting-Ting.,Zhu, Danmeng.,Chen, Wei.,Deng, Wei.,He, Hang.,...&Deng, Xing Wang.(2013).A Global Identification and Analysis of Small Nucleolar RNAs and Possible Intermediate-Sized Non-Coding RNAs in Oryza sativa.分子植物.
MLA	Liu, Ting-Ting,et al."A Global Identification and Analysis of Small Nucleolar RNAs and Possible Intermediate-Sized Non-Coding RNAs in Oryza sativa".分子植物 (2013).