Gene Network Analysis and Functional Studies of Senescence-associated   Genes Reveal Novel Regulators of Arabidopsis Leaf Senescence

doi:10.1111/j.1744-7909.2012.01136.x

CORC > 北京大学 > 生命科学学院

	Gene Network Analysis and Functional Studies of Senescence-associated Genes Reveal Novel Regulators of Arabidopsis Leaf Senescence
	Li, Zhonghai ; Peng, Jinying ; Wen, Xing ; Guo, Hongwei
刊名	journal of integrative plant biology
	2012
关键词	Arabidopsis leaf senescence SAG phytohormone systems biology DARK-INDUCED SENESCENCE PLANT SENESCENCE NITRIC-OXIDE TRANSCRIPTION FACTORS NEGATIVE REGULATORS SIGNALING PATHWAYS PATHOGEN-DEFENSE EXPRESSION ETHYLENE STRESS
DOI	10.1111/j.1744-7909.2012.01136.x
英文摘要	Plant leaf senescence has been recognized as the last phase of plant development, a highly ordered process regulated by genes known as senescence associated genes (SAGs). However, the function of most of SAGs in regulating leaf senescence as well as regulators of those functionally known SAGs are still unclear. We have previously developed a curated database of genes potentially associated with leaf senescence, the Leaf Senescence Database (LSD). In this study, we built gene networks to identify common regulators of leaf senescence in Arabidopsis thaliana using promoting or delaying senescence genes in LSD. Our results demonstrated that plant hormones cytokinin, auxin, nitric oxide as well as small molecules, such as Ca2+, delay leaf senescence. By contrast, ethylene, ABA, SA and JA as well as small molecules, such as oxygen, promote leaf senescence, altogether supporting the idea that phytohormones play a critical role in regulating leaf senescence. Functional analysis of candidate SAGs in LSD revealed that a WRKY transcription factor WRKY75 and a Cys2/His2type transcription factor AZF2 are positive regulators of leaf senescence and loss-of-function of WRKY75 or AZF2 delayed leaf senescence. We also found that silencing of a protein phosphatase, AtMKP2, promoted early senescence. Collectively, LSD can serve as a comprehensive resource for systematic study of the molecular mechanism of leaf senescence as well as offer candidate genes for functional analyses.; Biochemistry & Molecular Biology; Plant Sciences; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 23; ARTICLE; 8; 526-539; 54
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162501]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, Zhonghai,Peng, Jinying,Wen, Xing,et al. Gene Network Analysis and Functional Studies of Senescence-associated Genes Reveal Novel Regulators of Arabidopsis Leaf Senescence[J]. journal of integrative plant biology,2012.
APA	Li, Zhonghai,Peng, Jinying,Wen, Xing,&Guo, Hongwei.(2012).Gene Network Analysis and Functional Studies of Senescence-associated Genes Reveal Novel Regulators of Arabidopsis Leaf Senescence.journal of integrative plant biology.
MLA	Li, Zhonghai,et al."Gene Network Analysis and Functional Studies of Senescence-associated Genes Reveal Novel Regulators of Arabidopsis Leaf Senescence".journal of integrative plant biology (2012).