Induction of Pluripotency in Mouse Somatic Cells with Lineage Specifiers

doi:10.1016/j.cell.2013.05.001

CORC > 北京大学 > 生命科学学院

	Induction of Pluripotency in Mouse Somatic Cells with Lineage Specifiers
	Shu, Jian ; Wu, Chen ; Wu, Yetao ; Li, Zhiyuan ; Shao, Sida ; Zhao, Wenhui ; Tang, Xing ; Yang, Huan ; Shen, Lijun ; Zuo, Xiaohan ; Yang, Weifeng ; Shi, Yan ; Chi, Xiaochun ; Zhang, Hongquan ; Gao, Ge ; Shu, Youmin ; Yuan, Kehu ; He, Weiwu ; Tang, Chao ; Zhao, Yang ; Deng, Hongkui
刊名	cell
	2013
关键词	EMBRYONIC STEM-CELLS HUMAN FIBROBLASTS PRIMITIVE ENDODERM DIRECT CONVERSION GROUND-STATE SELF-RENEWAL DIFFERENTIATION GENERATION OCT4 EXPRESSION
DOI	10.1016/j.cell.2013.05.001
英文摘要	The reprogramming factors that induce pluripotency have been identified primarily from embryonic stem cell (ESC)-enriched, pluripotency-associated factors. Here, we report that, during mouse somatic cell reprogramming, pluripotency can be induced with lineage specifiers that are pluripotency rivals to suppress ESC identity, most of which are not enriched in ESCs. We found that OCT4 and SOX2, the core regulators of pluripotency, can be replaced by lineage specifiers that are involved in mesendo-dermal (ME) specification and in ectodermal (ECT) specification, respectively. OCT4 and its substitutes attenuated the elevated expression of a group of ECT genes, whereas SOX2 and its substitutes curtailed a group of ME genes during reprogramming. Surprisingly, the two counteracting lineage specifiers can synergistically induce pluripotency in the absence of both OCT4 and SOX2. Our study suggests a "seesaw model'' in which a balance that is established using pluripotency factors and/or counteracting lineage specifiers can facilitate reprogramming.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000319456800006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Cell Biology; SCI(E); PubMed; 104; ARTICLE; 5; 963-975; 153
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162479]
专题	生命科学学院
推荐引用方式 GB/T 7714	Shu, Jian,Wu, Chen,Wu, Yetao,et al. Induction of Pluripotency in Mouse Somatic Cells with Lineage Specifiers[J]. cell,2013.
APA	Shu, Jian.,Wu, Chen.,Wu, Yetao.,Li, Zhiyuan.,Shao, Sida.,...&Deng, Hongkui.(2013).Induction of Pluripotency in Mouse Somatic Cells with Lineage Specifiers.cell.
MLA	Shu, Jian,et al."Induction of Pluripotency in Mouse Somatic Cells with Lineage Specifiers".cell (2013).