DNA Barcoding of Rhodiola (Crassulaceae): A Case Study on a Group of   Recently Diversified Medicinal Plants from the Qinghai-Tibetan Plateau

doi:10.1371/journal.pone.0119921

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	DNA Barcoding of Rhodiola (Crassulaceae): A Case Study on a Group of Recently Diversified Medicinal Plants from the Qinghai-Tibetan Plateau
	Zhang, Jian-Qiang ; Meng, Shi-Yong ; Wen, Jun ; Rao, Guang-Yuan
刊名	plos one
	2015
关键词	LAND PLANTS SPECIES IDENTIFICATION CHLOROPLAST DNA TRNL-F SEQUENCES PHYLOGENY IDENTIFY MATK ZINGIBERACEAE LIKELIHOOD
DOI	10.1371/journal.pone.0119921
英文摘要	DNA barcoding, the identification of species using one or a few short standardized DNA sequences, is an important complement to traditional taxonomy. However, there are particular challenges for barcoding plants, especially for species with complex evolutionary histories. We herein evaluated the utility of five candidate sequences-rbcL, matK, trnH-psbA, trnL-F and the internal transcribed spacer (ITS) - for barcoding Rhodiola species, a group of high-altitude plants frequently used as adaptogens, hemostatics and tonics in traditional Tibetan medicine. Rhodiola was suggested to have diversified rapidly recently. The genus is thus a good model for testing DNA barcoding strategies for recently diversified medicinal plants. This study analyzed 189 accessions, representing 47 of the 55 recognized Rhodiola species in the Flora of China treatment. Based on intraspecific and interspecific divergence and degree of monophyly statistics, ITS was the best single-locus barcode, resolving 66% of the Rhodiola species. The core combination rbcL+matK resolved only 40.4% of them. Unsurprisingly, the combined use of all five loci provided the highest discrimination power, resolving 80.9% of the species. However, this is weaker than the discrimination power generally reported in barcoding studies of other plant taxa. The observed complications may be due to the recent diversification, incomplete lineage sorting and reticulate evolution of the genus. These processes are common features of numerous plant groups in the high-altitude regions of the Qinghai-Tibetan Plateau.; Multidisciplinary Sciences; SCI(E); PubMed; 0; ARTICLE; wenj@si.edu; rao@pku.edu.cn; 3; e0119921; 10
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162422]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang, Jian-Qiang,Meng, Shi-Yong,Wen, Jun,et al. DNA Barcoding of Rhodiola (Crassulaceae): A Case Study on a Group of Recently Diversified Medicinal Plants from the Qinghai-Tibetan Plateau[J]. plos one,2015.
APA	Zhang, Jian-Qiang,Meng, Shi-Yong,Wen, Jun,&Rao, Guang-Yuan.(2015).DNA Barcoding of Rhodiola (Crassulaceae): A Case Study on a Group of Recently Diversified Medicinal Plants from the Qinghai-Tibetan Plateau.plos one.
MLA	Zhang, Jian-Qiang,et al."DNA Barcoding of Rhodiola (Crassulaceae): A Case Study on a Group of Recently Diversified Medicinal Plants from the Qinghai-Tibetan Plateau".plos one (2015).