Label-free chemical imaging in vivo: three-dimensional non-invasive   microscopic observation of amphioxus notochord through stimulated Raman   scattering (SRS)

doi:10.1039/c2sc20316b

CORC > 北京大学 > 生命科学学院

	Label-free chemical imaging in vivo: three-dimensional non-invasive microscopic observation of amphioxus notochord through stimulated Raman scattering (SRS)
	Yu, Zhilong ; Chen, Tao ; Zhang, Xiannian ; Fu, Dan ; Liao, Xin ; Shen, Jie ; Liu, Xinxing ; Zhang, Bo ; Xie, X. Sunney ; Su, Xiao-Dong ; Chen, Junyuan ; Huang, Yanyi
刊名	chemical science
	2012
关键词	GREEN FLUORESCENT PROTEIN HIGH-SENSITIVITY CARS MICROSCOPY SINGLE MOLECULES LIPID DROPLETS BIOLOGY MICROSPECTROSCOPY SPECTROSCOPY GENERATION GENOME
DOI	10.1039/c2sc20316b
英文摘要	Notochord is a rod-shaped axial supporting structure unique only to chordates. In this study, we use cephalochordate amphioxus (Branchiostroma belcheri, a living basal chordate) and zebrafish (Danio rerio), a vertebrate, as model animals and employ stimulated Raman microscopy (SRS), a newly developed label-free technique, to investigate notochord structure and chemical composition in live animals. Coherent anti-Stokes Raman scattering (CARS) images have also been acquired for comparison. Both CARS and SRS images can construct the detailed three-dimensional structure of the notochord with resolution better than 1 mm. Label-free live imaging allows us to obtain the whole animal's intact internal morphology, which is difficult to obtain through other mechanical or optical sectioning methods. Intrinsically, chemical sensitive SRS images, with a simple data processing procedure, show that the amphioxus notochord is protein-rich. Our result agrees well with that drawn from the labeling and cryo-sectioning observation, verifying that the coherent Raman scattering techniques are useful to examine the structure and chemical composition of live animals without labels in a fast, simple and accurate way.; Chemistry, Multidisciplinary; SCI(E); EI; 0; ARTICLE; 8; 2646-2654; 3
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/154667]
专题	生命科学学院工学院
推荐引用方式 GB/T 7714	Yu, Zhilong,Chen, Tao,Zhang, Xiannian,et al. Label-free chemical imaging in vivo: three-dimensional non-invasive microscopic observation of amphioxus notochord through stimulated Raman scattering (SRS)[J]. chemical science,2012.
APA	Yu, Zhilong.,Chen, Tao.,Zhang, Xiannian.,Fu, Dan.,Liao, Xin.,...&Huang, Yanyi.(2012).Label-free chemical imaging in vivo: three-dimensional non-invasive microscopic observation of amphioxus notochord through stimulated Raman scattering (SRS).chemical science.
MLA	Yu, Zhilong,et al."Label-free chemical imaging in vivo: three-dimensional non-invasive microscopic observation of amphioxus notochord through stimulated Raman scattering (SRS)".chemical science (2012).