CHS基因起源初探及其在被子植物中的进化分析

doi:10.3321/j.issn:1672-9072.2004.01.002

CORC > 北京大学 > 生命科学学院

	CHS基因起源初探及其在被子植物中的进化分析; A Preliminary Study on the Origin and Evolution of Chalcone Synthase (CHS) Gene in Angiosperms
	黄金霞 ; 瞿礼嘉 ; 杨继 ; 银好 ; 顾红雅
刊名	植物学报
	2004
关键词	半月苔查尔酮合酶系统发育碱基替换速率 Lunularia cruciata chalcone synthase phylogeny substitution rate
DOI	10.3321/j.issn:1672-9072.2004.01.002
英文摘要	利用PCR与TAlL-PCR方法,从半月苔(Lunularia cructata(L.)Dum.ex Lindb)中获得了一段长约l 000 bp的基因片段,它与已知的CHS基因在核苷酸水平上的相似性大于56%,在氨基酸水平上的相似性大于60%,所推断的氨基酸序列中酶反应的4个催化位点与已知晶体结构的紫花苜蓿MCHS2A上的催化位点相同,首次证明了苔类植物中可能存在类CHS基因,将CHS基因的起源时间推到苔藓类植物出现之前.以该序列和两种蕨类植物(Psilotumnudum(L.)Griseb.和Equisetum arvense L.)的CHS序列作为外类群,应用邻接法、最大简约法和最大似然法分别构建了被子植物的CHS的分子系统树.结果表明,大部分科中的CHS分布在不同的分支上,而十字花科、可科和禾本科各自聚成一个单系类群.以邻接树为依据,对茄科、旋花科和菊科的CHS基因进行了相对碱基替换速率的检测,发现这三个科内或科间序列的替换速率不一致.被子植物的CHS基因在基因拷贝数目、碱基替换速率以及重复/丢失事件的发生上都存在较大的差异,这种差异可能与被子植物的生活史、生活环境、花的特性以及对外界的防御系统等的多样性相关.; By using Thermal Asymmetric Interlaced PCR (TAIL-PCR) method, a DNA fragment of about 1 000 bp was amplified and cloned from a liverwort species (Lunularia cruciata (L.) Dum. ex Lindb). The nucleotide sequence of this fragment and its deduced amino acid sequence shared about 56% and 60% identity with those of exon 2 of CHS genes from vascular plants respectively. The four characteristic catalyzing sites of CHS were found conserved in the deduced amino acid sequences of the fragment when compared with other CHS sequences. This is the first report of cloning a CHS-like gene from liverworts,suggesting that the origin of CHS genes may predate liverworts. Using the CHS-like sequence from L.cruciata and CHS sequences from two fern-alien species, Psilotum nudum (L.) Griseb. and Equisetum arvense L., as outgroups, the phylogenetic trees of about 250 CHSs from 29 families of angiosperm plants were constructed by using the neighbour-joining (NJ), maximum parsimony (MP) and quartet puzzle (QP)methods. The results showed that the CHSs from most plant families were separated into two or more clades while sequences from the families Brassicaceae, Fabaceae and Poaceae were each grouped into an independent monophyletic clade. The relative base substitution rates were estimated for CHS genes in three plant families, Solanaceae, Convolvulaceae, and Asteraceae, where the heterogeneity rate was detected both within and among the families. Results indicated that CHS genes in angiosperm plants were greatly diverse in terms of copy number, base substitution rate, and duplication/deletion events, which might be correlated with the diversity of life history, habitat, floral characters, and defense system of angiosperm plants.; 国家自然科学基金; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000189278000002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 7; 1; 10-19; 46
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/46983]
专题	生命科学学院
推荐引用方式 GB/T 7714	黄金霞,瞿礼嘉,杨继,等. CHS基因起源初探及其在被子植物中的进化分析, A Preliminary Study on the Origin and Evolution of Chalcone Synthase (CHS) Gene in Angiosperms[J]. 植物学报,2004.
APA	黄金霞,瞿礼嘉,杨继,银好,&顾红雅.(2004).CHS基因起源初探及其在被子植物中的进化分析.植物学报.
MLA	黄金霞,et al."CHS基因起源初探及其在被子植物中的进化分析".植物学报 (2004).