人脑枕叶区衰老进程的比较蛋白质组学研究

doi:10.3321/j.issn:0251-0790.2003.12.013

CORC > 北京大学 > 生命科学学院

	人脑枕叶区衰老进程的比较蛋白质组学研究; Comparative proteome analysis of human brain occipital lobes during the course of aging
	周波 ; 杨伟 ; 张景华 ; 童元鹏 ; 赵锐 ; 纪建国 ; 茹炳根
刊名	高等学校化学学报
	2003
关键词	蛋白质组双向凝胶电泳质谱人脑枕叶区衰老 proteome 2D-PAGE mass spectrometry human brain occipital lobes aging
DOI	10.3321/j.issn:0251-0790.2003.12.013
英文摘要	分别从 2 3岁、 64岁、 72岁、 83岁以及 94岁无神经性和精神性疾病史个体大脑皮层枕叶区取样 ,制备蛋白质样品 ,进行双向凝胶电泳 ( 2 -DE)、考马斯亮蓝染色、凝胶扫描和 Image Master2 D Elite软件分析 ,每张胶上平均可检测到 1 0 0 0个以上蛋白质点 .通过软件半定量分析 ,进一步研究了衰老过程中枕叶蛋白质的差异表达 ,发现随年龄增长有 7种蛋白质有一致的显著上升或下降趋势 .应用质谱进行肽质量指纹图谱( PMF)和 /或肽序列标签 ( PST)分析 ,数据库检索共鉴定了 1 1种蛋白质 ,其中有 5种具有一致的上调或下调性 ,包括神经元突触结构蛋白低...; Proteomic analysis is a powerful tool to reveal the mechanism of functional changes of central nervous system and the origination, development and prognosis of central nervous disease. Human brain proteins were isolated from human occipital lobes at five ages from 23 to 94 years old, and separated by two dimensional gel electrophoresis. Over one thousand protein spots were resolved in one gel with commassic brilliant blue staining. Differential expression of proteins with increasing ages was analyzed by Image Master 2D Elite software processing. Seven protein spots were found to increase or decrease with increasing ages consistently. Taking advantage of the high sensitivity and accuracy of mass spectrometry, 11 proteins with significant changes were identified by peptide fingerprinting map(PMF) or peptide sequence tag (PST) obtained from MS or tandem MS (MS/MS). Among the identified proteins, the amount of 5 proteins were increased or decreased consistently, including neurofilament triplet L protein(NF-L), a protein involved in the maintenance of neuronal caliber; peroxiredoxin(EC 1. 11. 1.), an antioxidant enzyme; aconitate hydratase(EC 4. 2. 1. 3), a key,enzyme in tricarboxylic acid(TCA) cycle; enolase 2(EC 4. 2. 1. 11), a key enzyme in glycolysis pathway; T-complex protein 1, a chaperone. In summary, the two dimensional map of human brain occipital lobe proteins was firstly established, significantly changed proteins during the course of aging were identified and their possible biological significance was discussed.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000187660500017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 5; 12; 2202-2207; 24
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/46811]
专题	生命科学学院
推荐引用方式 GB/T 7714	周波,杨伟,张景华,等. 人脑枕叶区衰老进程的比较蛋白质组学研究, Comparative proteome analysis of human brain occipital lobes during the course of aging[J]. 高等学校化学学报,2003.
APA	周波.,杨伟.,张景华.,童元鹏.,赵锐.,...&茹炳根.(2003).人脑枕叶区衰老进程的比较蛋白质组学研究.高等学校化学学报.
MLA	周波,et al."人脑枕叶区衰老进程的比较蛋白质组学研究".高等学校化学学报 (2003).