杜仲次生木质部细胞分化和脱分化过程中ATPase的超微细胞化学定位(英文)

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	杜仲次生木质部细胞分化和脱分化过程中ATPase的超微细胞化学定位(英文); Ultracytochemical localization of ATPase during the secondary xylem differentiation and dedifferentiation in Eucommia ulmoides trunk
	王雅清 ; 崔克明 ; Kalima-N&apos
刊名	植物学报
	2000
关键词	杜仲次生木质部分化脱分化细胞程序性死亡(PCD) ATPase Eucommia ulmoides secondary xylem differentiation dedifferentiation programmed cell death (PCD) ATPase
英文摘要	采用磷酸铅沉淀技术 ,对杜仲 (EucommiaulmoidesOliv .)次生木质部细胞分化和脱分化过程进行了ATPase的超微细胞化学定位。随着分化过程中细胞程序性死亡 (programmedcelldeath ,PCD)程度的加深 ,ATPase在细胞核上的分布由少变多 ,而在各种细胞器上的分布由有到无 ,并且随着细胞质的解体 ,ATPase在细胞壁内侧和纹孔处的分布也由少到多 ,说明它们的变化是由核基因控制的主动需能过程 ;杜仲剥皮后的脱分化过程中 ,随着细胞开始分裂进而迅速分裂形成新的形成层 ,胞间隙和胞间层中ATPase的分布由少到多 ,但分裂细胞的核上无此酶分布 ,可能细胞由分...; The ultracytochemical localization of ATPase in the secondary xylem cells during their differentiation and dedifferentiation in the girdled Eucommia ulmoides Oliv. was carried out using a lead phosphate precipitation technique. Throughout the differentiation, which is a typical programmed cell death (PCD) process, ATPase deposits increased in the nucleus hut decreased and progressively disappeared in the cell organelles. At the same time, the distribution of ATPase increased in the inner face of the cell wall and pits with cytoplasmic degeneration. The results demonstrated that the PCD was an energy dependent active process and was controlled by nuclear genes. On the other hand, the distribution of ATPase in the intercellular spaces increased with the formation of the new cambium resulted from the dedifferentiation of the secondary xylem cells after girdling. However, ATPase tvas not found in the nucleus of the dividing cells, suggesting that nutrients were transported through protoplast during differentiation, and through both protoplast and apoplast during dedifferentiation. Thus, the energy required in cell division was provided mainly by intercellular spaces. These findings indicate that the dynamic distribution of ATPase reflected which cell component was actively taking part in the cell metabolism at various stages of the plant development, and its distribution was associated with the physiological state of the cell. Based on the characteristic distributions of ATPase, the critical stage of cell differentiation and the relationship between the critical stage and dedifferentiation were discussed.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000087268900003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中文核心期刊要目总览(PKU); 中国科学引文数据库(CSCD); 2; 05; 455-460; 42
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/45936]
专题	生命科学学院
推荐引用方式 GB/T 7714	王雅清,崔克明,Kalima-N&apos. 杜仲次生木质部细胞分化和脱分化过程中ATPase的超微细胞化学定位(英文), Ultracytochemical localization of ATPase during the secondary xylem differentiation and dedifferentiation in Eucommia ulmoides trunk[J]. 植物学报,2000.
APA	王雅清,崔克明,&Kalima-N&apos.(2000).杜仲次生木质部细胞分化和脱分化过程中ATPase的超微细胞化学定位(英文).植物学报.
MLA	王雅清,et al."杜仲次生木质部细胞分化和脱分化过程中ATPase的超微细胞化学定位(英文)".植物学报 (2000).