生长抑制因子(GIF)与G蛋白Rab3a直接相互作用

doi:10.3321/j.issn:1000-3282.2001.06.026

CORC > 北京大学 > 生命科学学院

	生长抑制因子(GIF)与G蛋白Rab3a直接相互作用; Growth inhibitory factor (GIF) directly interacts with G-protein Rab3a
	康巧华 ; 陈巧林 ; 任宏伟 ; 茹炳根
刊名	生物化学与生物物理进展
	2001
关键词	生长抑制因子阿尔茨海默氏症酵母双杂交系统免疫共沉淀 Rab3a growth inhibitory factor Alzheimer&apos yeast two-hybrid system Rab3a coimmunoprecipitation s disease
DOI	10.3321/j.issn:1000-3282.2001.06.026
英文摘要	生长抑制因子 (growthinhibitoryfactor,GIF) ,又称金属硫蛋白 3,为 6 8个氨基酸组成的脑特异性金属硫蛋白 ,具有广泛的生理功能 ;GIF可能与阿尔茨海默氏症 (Alzheimer s)病理相关 ,在Alzheimer s脑提取物存在下 ,还对神经细胞具有特异的生长抑制活性 .然而 ,对其发挥生长抑制作用的分子机制并不清楚 .运用酵母双杂交系统从人脑cDNA文库中筛选与GIF相互作用因子 ,从 4 1× 10 6个人脑cDNA文库转化子中 ,首次筛选到Ras家族G蛋白Rab3aC端 ,包含 87个氨基酸的片段能与GIF相互作用 ;用PCR自人胎盘总cDNA中获得包...; Growth inhibitory factor (GIF), renamed as metallothionein-3, is a 68-amino acids brain specific member of the metallothionein family. GIF plays comprehensively physiologic roles and is deemed to be implicated in Alzheimer's disease. When supplemented with Alzheimer's brain extracts, GIF demonstrates its in vitro growth inhibitory activity on cultured neuron; but the underlying molecular mechanism remains unknown. To probe this mystery, the yeast two-hybrid system was employed to screen a human brain cDNA library for GIF-interacting proteins. From 4.1 x 10(6) transformants of library screening, a C-terminal fragment of small G-protein Rab3a was obtained, harboring 87 amino acids that interacted with GIF in yeast. The full-length Rab3a was amplified by polymerase chain reaction ( PCR) using human total placenta cDNA as template ana concomitantly cloned. In yeast two-hybrid test, full-length Rab3a also interacted with GIF. Through coimmunoprecipitation and Western blotting experiments, interaction between GIF and Rab3a was confirmed in mammalian cell. Furthermore, the result indicated that Rab3a interacted with GIF in a GTP-binding form.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000172947500026&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中文核心期刊要目总览(PKU); 中国科学引文数据库(CSCD); 11; 06; 880-884; 28
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/45879]
专题	生命科学学院
推荐引用方式 GB/T 7714	康巧华,陈巧林,任宏伟,等. 生长抑制因子(GIF)与G蛋白Rab3a直接相互作用, Growth inhibitory factor (GIF) directly interacts with G-protein Rab3a[J]. 生物化学与生物物理进展,2001.
APA	康巧华,陈巧林,任宏伟,&茹炳根.(2001).生长抑制因子(GIF)与G蛋白Rab3a直接相互作用.生物化学与生物物理进展.
MLA	康巧华,et al."生长抑制因子(GIF)与G蛋白Rab3a直接相互作用".生物化学与生物物理进展 (2001).