木醋杆菌纤维素合成操纵子的克隆及棉花转化(英文)

doi:10.3321/j.issn:1672-9072.2002.04.011

CORC > 北京大学 > 生命科学学院

	木醋杆菌纤维素合成操纵子的克隆及棉花转化(英文); Cloning whole cellulose-synthesizing operon (ayacs operon) from Acetobacter xylinum and transforming it into cultivated cotton plants
	卢迎春 ; 魏刚 ; 朱玉贤
刊名	植物学报
	2002
关键词	木醋杆菌纤维素合成操纵子棉花转化 Acetobacter xylinum cellulose-synthesizing operon cotton transformation
DOI	10.3321/j.issn:1672-9072.2002.04.011
英文摘要	革兰氏阴性菌木醋杆菌 (Acetobacterxylinum (Brown)Yamada)合成一种由纤维素微纤丝组成的胞外带状物。与高等植物纤维素相比 ,它具有独特的结构和机械性能。根据从木醋杆菌ATCC 5 35 82克隆的acs纤维素合成操纵子序列设计引物 ,用PCR的方法从木醋杆菌Ay2 0 1中克隆了ayacs纤维素合成操纵子的全部 4个基因。序列比较发现 ,两者高度同源。将连上CaMV 35S启动子的acsA、acsB克隆到植物表达载体pCAMBIA 130 1上 ,acsC、acsD克隆到pCOB30 2_3中。然后通过花粉管通道法转化棉花 (Gossypiumhirsutum)胚...; The gram-negative bacterium Acetobacter xylinum synthesizes an extracellular ribbon of cellulose microfibrils that possess unique structural and mechanical properties when compared to higher plant cellulose. All four genes in the cellulose-synthesizing operon (ayacs operon) of A. xylinum Ay201 were amplified by polymerase chain reaction (PCR) using oligonucleotide primers designed according to published acs operon sequence of A. xylinum ATCC 53582. Alignment of the two operons showed that they were highly homologous (98% similarity, 97% identity). AcsA and acsB gene were cloned in pCAMBIA 1301 vector while acsC and acsD were cloned in pCOB302-3 under the control of CaM 35S promoter. The constructs were introduced into cotton by the pollen-tube-pathway method and seeds obtained from putative transgenic plants were germinated on media containing hygromycin and phosphinothricin (PPT). Five seedlings out of 934 seeds were proved to contain all four foreign genes by PCR amplification. This is the first time that a whole operon encoding four different bacterial enzymes with various biological functions is transformed into cultivated cotton plants.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000175057100011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中国科学引文数据库(CSCD); 2; 04; 441-445; 44
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/45831]
专题	生命科学学院
推荐引用方式 GB/T 7714	卢迎春,魏刚,朱玉贤. 木醋杆菌纤维素合成操纵子的克隆及棉花转化(英文), Cloning whole cellulose-synthesizing operon (ayacs operon) from Acetobacter xylinum and transforming it into cultivated cotton plants[J]. 植物学报,2002.
APA	卢迎春,魏刚,&朱玉贤.(2002).木醋杆菌纤维素合成操纵子的克隆及棉花转化(英文).植物学报.
MLA	卢迎春,et al."木醋杆菌纤维素合成操纵子的克隆及棉花转化(英文)".植物学报 (2002).