高效建立129/ter,C57BL/6J小鼠胚胎干细胞系的方法学探讨

doi:10.3321/j.issn:1000-3061.2002.06.018

CORC > 北京大学 > 生命科学学院

	高效建立129/ter,C57BL/6J小鼠胚胎干细胞系的方法学探讨; Discussion of the Methods for Establishing Embryonic Stem Cell Lines from 129/ter、C57BL/6J Mice with High Efficiency
	孟国良 ; 汤富酬 ; 尚克刚 ; 薛友纺
刊名	生物工程学报
	2002
关键词	129/ter小鼠,C57BL/6J小鼠,大鼠心脏细胞条件培养(RH-CM),胚胎干细胞(ES细胞),二倍体核型,未分化状态
DOI	10.3321/j.issn:1000-3061.2002.06.018
英文摘要	小鼠胚胎干细胞(ES细胞)是从小鼠囊胚内细胞团(ICM)分离出来的、在体外培养过程中可维持未分化状态、正常二倍体核型及无限增殖能力，具有多能性或全能性的细胞系[1-3]。; A new method for establishing ES cell lines from 129/ter. C57BL/6J mice was set up which was characterized by the murine embryonic fibroblast cell(MEF) feeder, the medium of rat heart cell-conditioned medium(RH-CM) for ES cells, and the consecutive digestion by the digestion liquid containing 1% serum. Every group of improved experiments was done with a control of routine method. The results showed that, compared with routine method, the improved way increased the ratio of ES cell lines of 129/ter mice from 11.8% to 33.3%, and of C57BL/6J from 3.7% to 13.3%. The difference is distinct. The passage culture of ES cells showed that, compared with medium added LIF, RH-CM not only inhibited the differentiation of murine ES cells, maintained its dipoild karyotype, but also promote its adherence growth. This kind of culture condition not only maintained the ES cells in an undifferentiated state and their normal dipoild karyotype, but also a series of other characteristics of totipotent embryonic stem cells during extended culture period.; 国家自然科学基金; PubMed; 中文核心期刊要目总览(PKU); 中国科学引文数据库(CSCD); 0; 6; 740-743; 18
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/45599]
专题	生命科学学院
推荐引用方式 GB/T 7714	孟国良,汤富酬,尚克刚,等. 高效建立129/ter,C57BL/6J小鼠胚胎干细胞系的方法学探讨, Discussion of the Methods for Establishing Embryonic Stem Cell Lines from 129/ter、C57BL/6J Mice with High Efficiency[J]. 生物工程学报,2002.
APA	孟国良,汤富酬,尚克刚,&薛友纺.(2002).高效建立129/ter,C57BL/6J小鼠胚胎干细胞系的方法学探讨.生物工程学报.
MLA	孟国良,et al."高效建立129/ter,C57BL/6J小鼠胚胎干细胞系的方法学探讨".生物工程学报 (2002).