Magnetization of 3-dimentional homochiral metal-organic frameworks for   efficient and highly selective capture of phosphopeptides

doi:10.1016/j.chroma.2016.09.046

CORC > 北京大学 > 化学与分子工程学院

	Magnetization of 3-dimentional homochiral metal-organic frameworks for efficient and highly selective capture of phosphopeptides
	Qi, Xiaoyue ; Chang, Cuilan ; Xu, Xinyuan ; Zhang, Yiding ; Bai, Yu ; Liu, Huwei
刊名	JOURNAL OF CHROMATOGRAPHY A
	2016
关键词	Affinity probes Phosphopeptides Enrichment Chiral MOFs FACILE SYNTHESIS ENRICHMENT GRAPHENE PHOSPHORYLATION NANOPARTICLES MICROSPHERES MECHANISM PLATFORM CANCER
DOI	10.1016/j.chroma.2016.09.046
英文摘要	Enrichment of phosphopeptides based on various affinity probes prior to mass spectrometry detection is usually required due to the low abundance and low ionization efficiency of phosphopeptides. In this work, a 3-dimentional homochiral metal-organic frameworks (MOFs) was modified with magnetic nanoparticles using a facile method and then utilized for phosphopeptides capture with high efficiency and specificity. Based on magnetic solid phase extraction, a rapid and efficient method was developed and the whole enrichment procedure could be easily finished within 10 min. Efficient and highly selective capture of phosphopeptides from tryptic digests and human serum was achieved. This affinity probe showed satisfactory reproducibility of the particle synthesis and could be recycled for at least seven times. With all the advantages mentioned above, this strategy is of great potential for routine application in phosphoproteomes. (C) 2016 Elsevier B.V. All rights reserved.; National Natural Science Foundation of China [21322505, 21175008]; SCI(E); PubMed; ARTICLE; hwliu@pku.edu.cn; 49-54; 1468
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/492801]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Qi, Xiaoyue,Chang, Cuilan,Xu, Xinyuan,et al. Magnetization of 3-dimentional homochiral metal-organic frameworks for efficient and highly selective capture of phosphopeptides[J]. JOURNAL OF CHROMATOGRAPHY A,2016.
APA	Qi, Xiaoyue,Chang, Cuilan,Xu, Xinyuan,Zhang, Yiding,Bai, Yu,&Liu, Huwei.(2016).Magnetization of 3-dimentional homochiral metal-organic frameworks for efficient and highly selective capture of phosphopeptides.JOURNAL OF CHROMATOGRAPHY A.
MLA	Qi, Xiaoyue,et al."Magnetization of 3-dimentional homochiral metal-organic frameworks for efficient and highly selective capture of phosphopeptides".JOURNAL OF CHROMATOGRAPHY A (2016).