Genetically Encoded Photocrosslinkers for Identifying and Mapping   Protein-Protein Interactions in Living Cells

doi:10.1002/iub.1560

CORC > 北京大学 > 化学与分子工程学院

	Genetically Encoded Photocrosslinkers for Identifying and Mapping Protein-Protein Interactions in Living Cells
	Yang, Yi ; Song, Haiping ; Chen, Peng R.
刊名	IUBMB LIFE
	2016
关键词	photocrosslinker genetic code expansion protein-protein interactions interaction dynamics interaction interface PHOTO-CROSS-LINKING TRANSFER-RNA SYNTHETASE PHOTOREACTIVE AMINO-ACID MASS-SPECTROMETRY ESCHERICHIA-COLI MAMMALIAN-CELLS CODE LIPOPOLYSACCHARIDE COMPLEXES PHENYLALANINE
DOI	10.1002/iub.1560
英文摘要	Protein-protein interactions (PPIs) play pivotal roles in regulation of many biological processes. Conventional methods are capable of investigating stable and strong interactions within protein complexes, but remain difficult for studying dynamic, transient, and weak PPIs. Herein we review photo-affinity unnatural amino acids that can be site-specifically incorporated into a protein of interest to covalently trap non-covalent PPIs under living conditions. A newly developed cleavable photocrosslinker from our group will also be introduced, which facilitated the prey-bait separation for better enrichment and identification of photocrosslinked PPI complexes. (C) 2016 IUBMB Life; National Basic Research Program of China [2012CB917301]; SCI(E); PubMed; REVIEW; pengchen@pku.edu.cn; 11,SI; 879-886; 68
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/492706]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Yang, Yi,Song, Haiping,Chen, Peng R.. Genetically Encoded Photocrosslinkers for Identifying and Mapping Protein-Protein Interactions in Living Cells[J]. IUBMB LIFE,2016.
APA	Yang, Yi,Song, Haiping,&Chen, Peng R..(2016).Genetically Encoded Photocrosslinkers for Identifying and Mapping Protein-Protein Interactions in Living Cells.IUBMB LIFE.
MLA	Yang, Yi,et al."Genetically Encoded Photocrosslinkers for Identifying and Mapping Protein-Protein Interactions in Living Cells".IUBMB LIFE (2016).