Dimension conversion and scaling of disordered protein chains

doi:10.1039/c6mb00415f

CORC > 北京大学 > 化学与分子工程学院

	Dimension conversion and scaling of disordered protein chains
	Li, Maodong ; Sun, Tanlin ; Jin, Fan ; Yu, Daqi ; Liu, Zhirong
刊名	MOLECULAR BIOSYSTEMS
	2016
关键词	SINGLE-MOLECULE SPECTROSCOPY UNFOLDED PROTEINS NONNATIVE INTERACTIONS FOLDING SIMULATIONS INTRINSIC DISORDER GLOBULE TRANSITION DESIGNED PROTEIN TRANSFER MODEL STATE FRET
DOI	10.1039/c6mb00415f
英文摘要	To extract protein dimension and energetics information from single-molecule fluorescence resonance energy transfer spectroscopy (smFRET) data, it is essential to establish the relationship between the distributions of the radius of gyration (R-g) and the end-to-end (donor-to-acceptor) distance (R-ee). Here, we performed a coarse-grained molecular dynamics simulation to obtain a conformational ensemble of denatured proteins and intrinsically disordered proteins. For any disordered chain with fixed length, there is an excellent linear correlation between the average values of R-g and R-ee under various solvent conditions, but the relationship deviates from the prediction of a Gaussian chain. A modified conversion formula was proposed to analyze smFRET data. The formula reduces the discrepancy between the results obtained from FRET and small-angle X-ray scattering (SAXS). The scaling law in a coil-globule transition process was examined where a significant finite-size effect was revealed, i.e., the scaling exponent may exceed the theoretical critical boundary [1/3, 3/5] and the prefactor changes notably during the transition. The Sanchez chain model was also tested and it was shown that the mean-field approximation works well for expanded chains.; Ministry of Science and Technology of China [2015CB910300]; SCI(E); PubMed; ARTICLE; LiuZhiRong@pku.edu.cn; 9; 2932-2940; 12
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/492451]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Li, Maodong,Sun, Tanlin,Jin, Fan,et al. Dimension conversion and scaling of disordered protein chains[J]. MOLECULAR BIOSYSTEMS,2016.
APA	Li, Maodong,Sun, Tanlin,Jin, Fan,Yu, Daqi,&Liu, Zhirong.(2016).Dimension conversion and scaling of disordered protein chains.MOLECULAR BIOSYSTEMS.
MLA	Li, Maodong,et al."Dimension conversion and scaling of disordered protein chains".MOLECULAR BIOSYSTEMS (2016).