Toxicological Effects of Caco-2 Cells Following Short-Term and Long-Term   Exposure to Ag Nanoparticles

doi:10.3390/ijms17060974

CORC > 北京大学 > 化学与分子工程学院

	Toxicological Effects of Caco-2 Cells Following Short-Term and Long-Term Exposure to Ag Nanoparticles
	Chen, Ni ; Song, Zheng-Mei ; Tang, Huan ; Xi, Wen-Song ; Cao, Aoneng ; Liu, Yuanfang ; Wang, Haifang
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2016
关键词	Ag nanoparticle Caco-2 cells surface coating long-term low-dose toxicity TITANIUM-DIOXIDE NANOPARTICLES COATED SILVER NANOPARTICLES IN-VITRO EXPOSURE CELLULAR UPTAKE GOLD NANOPARTICLES OXIDATIVE STRESS CYTOTOXICITY TOXICITY SIZE GENOTOXICITY
DOI	10.3390/ijms17060974
英文摘要	Extensive utilization increases the exposure of humans to Ag nanoparticles (NPs) via the oral pathway. To comprehensively address the action of Ag NPs to the gastrointestinal systems in real situations, i.e., the long-term low-dose exposure, we evaluated and compared the toxicity of three Ag NPs (20-30 nm with different surface coatings) to the human intestine cell Caco-2 after 1-day and 21-day exposures, using various biological assays. In both the short-and long-term exposures, the variety of surface coating predominated the toxicity of Ag NPs in a descending order of citrate-coated Ag NP (Ag-CIT), bare Ag NP (Ag-B), and poly (N-vinyl-2-pyrrolidone)-coated Ag NP (Ag-PVP). The short-term exposure induced cell growth inhibition and death. The cell viability loss appeared after cells were exposed to 0.7 mu g/mL Ag-CIT, 0.9 mu g/mL Ag-B or >1.0 mu g/mL Ag-PVP for 24 h. The short-term and higher-dose exposure also induced reactive oxygen species (ROS) generation, mitochondrial damage, cell membrane leakage, apoptosis, and inflammation (IL-8 level). The long-term exposure only inhibited the cell proliferation. After 21-day exposure to 0.4 mu g/mL Ag-CIT, the cell viability dropped to less than 50%, while cells exposed to 0.5 mu g/mL Ag-PVP remained normal as the control. Generally, 0.3 mu g/mL is the non-toxic dose for the long-term exposure of Caco-2 cells to Ag NPs in this study. However, cells presented inflammation after exposure to Ag NPs with the non-toxic dose in the long-term exposure.; National Basic Research Program of China (973 Program) [2011CB933402]; Chinese Natural Science Foundation [21371117, 31571024]; SCI(E); PubMed; ARTICLE; chenni2686@163.com; szmeismile@126.com; huantang@pku.edu.cn; xwsgsy@126.com; ancao@shu.edu.cn; hwang@shu.edu.cn; hwang@shu.edu.cn; 6; 17
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/492264]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Chen, Ni,Song, Zheng-Mei,Tang, Huan,et al. Toxicological Effects of Caco-2 Cells Following Short-Term and Long-Term Exposure to Ag Nanoparticles[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016.
APA	Chen, Ni.,Song, Zheng-Mei.,Tang, Huan.,Xi, Wen-Song.,Cao, Aoneng.,...&Wang, Haifang.(2016).Toxicological Effects of Caco-2 Cells Following Short-Term and Long-Term Exposure to Ag Nanoparticles.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES.
MLA	Chen, Ni,et al."Toxicological Effects of Caco-2 Cells Following Short-Term and Long-Term Exposure to Ag Nanoparticles".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2016).