Shear Effects on Stability of DNA Complexes in the Presence of Serum

doi:10.1021/acs.biomac.7b00900

CORC > 北京大学 > 化学与分子工程学院

	Shear Effects on Stability of DNA Complexes in the Presence of Serum
	Wen, Hao ; Yu, Quhong ; Yin, Yudan ; Pan, Wei ; Yang, Shuang ; Liang, Dehai
刊名	BIOMACROMOLECULES
	2017
关键词	POLYELECTROLYTE COMPLEXES DROP DEFORMATION DRUG-DELIVERY PROTEIN ADSORPTION CATIONIC POLYMERS CELLULAR UPTAKE SURFACE-CHARGE NANOPARTICLES BREAKUP FLOW
DOI	10.1021/acs.biomac.7b00900
英文摘要	The behavior of nanocarriers, even though they are well-defined at equilibrium conditions, is unpredictable in living system. Using the complexes formed by plasmid DNA (pDNA) and K-20 (K: lysine), protamine, or polylysine (PLL) as models, we studied the dynamic behavior of gene carriers in the presence of fetal bovine serum (FBS) and under different shear rates, a condition mimicking the internal physical environment of blood vessels. Without shear, all the positively charged complexes bind to the negatively charged proteins in FBS, leading to the formation of large aggregates and even precipitates. The behaviors are quite different under shear. The shear generates two effects: a mechanical force to break down the complex into smaller size particles above a critical shear rate and a stirring effect leading to secondary aggregation of complexes below the critical shear rate. In the studied shear rate from 100 to 3000 s(-1), the mechanical force plays a key role in K-20/pDNA and protamine/pDNA, while the stirring effect is dominant in PLL/pDNA. A model study shows that the interfacial tension, the chain density, and the elasticity of the complexes determine their responsiveness to shear force. This study is helpful to understand the fate of drug/gene carriers under physiological conditions. It also gains insight in designing drug/gene carriers with desirable properties for in vivo applications.; National Natural Science Foundation of China [21574002]; Beijing Natural Science Foundation [2171001]; SCI(E); ARTICLE; 10; 3252-3259; 18
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/484820]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wen, Hao,Yu, Quhong,Yin, Yudan,et al. Shear Effects on Stability of DNA Complexes in the Presence of Serum[J]. BIOMACROMOLECULES,2017.
APA	Wen, Hao,Yu, Quhong,Yin, Yudan,Pan, Wei,Yang, Shuang,&Liang, Dehai.(2017).Shear Effects on Stability of DNA Complexes in the Presence of Serum.BIOMACROMOLECULES.
MLA	Wen, Hao,et al."Shear Effects on Stability of DNA Complexes in the Presence of Serum".BIOMACROMOLECULES (2017).