Epitranscriptome sequencing technologies: decoding RNA modifications | |
Li, Xiaoyu ; Xiong, Xushen ; Yi, Chengqi | |
刊名 | NATURE METHODS |
2017 | |
关键词 | DYNAMIC N-1-METHYLADENOSINE METHYLOME SINGLE-NUCLEOTIDE-RESOLUTION MESSENGER-RNA HUMAN TRANSCRIPTOME WIDESPREAD RNA EDITING SITES RIBOSOMAL-RNA GENE-EXPRESSION NUCLEAR-RNA MAPPING REVEALS |
DOI | 10.1038/NMETH.4110 |
英文摘要 | In recent years, major breakthroughs in RNA-modification-mediated regulation of gene expression have been made, leading to the emerging field of epitranscriptomics. Our understanding of the distribution, regulation and function of these dynamic RNA modifications is based on sequencing technologies. In this Review, we focus on the major mRNA modifications in the transcriptome of eukaryotic cells: N-6-methyladenosine, N-6,2'-0-dimethyladenosine, 5-methylcytidine, 5-hydroxylmethylcytidine, inosine, pseudouridine and N-1-methyladenosine. We discuss the sequencing technologies used to profile these epitranscriptomic marks, including scale, resolution, quantitative feature, pre-enrichment capability and the corresponding bioinformatics tools. We also discuss the challenges of epitranscriptome profiling and highlight the prospect of future detection tools. We aim to guide the choice of different detection methods and inspire new ideas in RNA biology.; National Basic Research Foundation of China [MOST2016YFC0900300, 2014CB964900]; National Natural Science Foundation of China [21522201]; SCI(E); REVIEW; 1; 23-31; 14 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/476495] |
专题 | 化学与分子工程学院 生命科学学院 |
推荐引用方式 GB/T 7714 | Li, Xiaoyu,Xiong, Xushen,Yi, Chengqi. Epitranscriptome sequencing technologies: decoding RNA modifications[J]. NATURE METHODS,2017. |
APA | Li, Xiaoyu,Xiong, Xushen,&Yi, Chengqi.(2017).Epitranscriptome sequencing technologies: decoding RNA modifications.NATURE METHODS. |
MLA | Li, Xiaoyu,et al."Epitranscriptome sequencing technologies: decoding RNA modifications".NATURE METHODS (2017). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论