Salmonella proteomics under oxidative stress reveals coordinated   regulation of antioxidant defense with iron metabolism and bacterial   virulence

doi:10.1016/jjprot.2017.02.004

CORC > 北京大学 > 化学与分子工程学院

	Salmonella proteomics under oxidative stress reveals coordinated regulation of antioxidant defense with iron metabolism and bacterial virulence
	Fu, Jiaqi ; Qi, Linlu ; Hu, Mo ; Liu, Yanhua ; Yu, Kaiwen ; Liu, Qian ; Liu, Xiaoyun
刊名	JOURNAL OF PROTEOMICS
	2017
关键词	Salmonella proteomics Oxidative stress Antioxidant defense Iron metabolism Bacterial virulence ENTERICA SEROVAR TYPHIMURIUM ESCHERICHIA-COLI HYDROGEN-PEROXIDE SUPEROXIDE-DISMUTASE HEAT-SHOCK PROTEIN DAMAGE IDENTIFICATION MUTAGENESIS RESISTANCE
DOI	10.1016/jjprot.2017.02.004
英文摘要	Salmonella Typhimurium is a bacterial pathogen that can cause widespread gastroenteritis. Salmonella encounters reactive oxygen species both under free-living conditions and within their mammalian host during infection. To study its response to oxidative stress, we performed the first large-scale proteomic profiling of Salmonella upon exposure to H2O2. Among 1600 detected proteins, 83 proteins showed significantly altered abundance. Interestingly, only a subset of known antioxidants was induced, likely due to distinct regulatory mechanisms. In addition, we found elevation of several Salmonella acquired phage products with potential contribution to DNA repair under oxidative stress. Furthermore, we observed robust induction of iron-uptake systems and disruption of these pathways led to bacterial survival defects under H2O2 challenge. Importantly, this work is the first to report that oxidative stress severely repressed the Salmonella type III secretion system (T3SS), reducing its virulence. Biological significance Salmonella, a Gram-negative bacterial pathogen, encounters reactive oxygen species (ROS) both endogenously and exogenously. To better understand its response to oxidative stress, we performed the first large-scale profiling of Salmonella protein expression upon H2O2 treatment. Among 1600 quantified proteins, the abundance of 116 proteins was altered significantly. Notably, iron acquisition systems were induced to promote bacterial survival under oxidative stress. Furthermore, we are the first to report that oxidative stress severely repressed Salmonella type III secretion system and hence reduced its virulence. We believe that these findings will not only help us better understand the molecular mechanisms that Salmonella has evolved to counteract ROS but also the global impact of oxidative stress on bacterial physiology. (C) 2017 Elsevier B.V. All rights reserved.; National Natural Science Foundation of China [21622501, 21475005]; Thousand Young Talents program of the Chinese government; SCI(E); ARTICLE; 52-58; 157
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/474507]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Fu, Jiaqi,Qi, Linlu,Hu, Mo,et al. Salmonella proteomics under oxidative stress reveals coordinated regulation of antioxidant defense with iron metabolism and bacterial virulence[J]. JOURNAL OF PROTEOMICS,2017.
APA	Fu, Jiaqi.,Qi, Linlu.,Hu, Mo.,Liu, Yanhua.,Yu, Kaiwen.,...&Liu, Xiaoyun.(2017).Salmonella proteomics under oxidative stress reveals coordinated regulation of antioxidant defense with iron metabolism and bacterial virulence.JOURNAL OF PROTEOMICS.
MLA	Fu, Jiaqi,et al."Salmonella proteomics under oxidative stress reveals coordinated regulation of antioxidant defense with iron metabolism and bacterial virulence".JOURNAL OF PROTEOMICS (2017).