A specific DNA-nanoprobe for tracking the activities of human   apurinic/apyrimidinic endonuclease 1 in living cells

doi:10.1093/nar/gkw1205

CORC > 北京大学 > 化学与分子工程学院

	A specific DNA-nanoprobe for tracking the activities of human apurinic/apyrimidinic endonuclease 1 in living cells
	Zhai, Junqiu ; Liu, Yibin ; Huang, Shan ; Fang, Simin ; Zhao, Meiping
刊名	NUCLEIC ACIDS RESEARCH
	2017
关键词	BASE EXCISION-REPAIR MODIFIED SILICA NANOPARTICLES GENE DELIVERY DRUG-DELIVERY APE1/REF-1 PROTEIN APE1 NANOCARRIERS EXPRESSION TARGET
DOI	10.1093/nar/gkw1205
英文摘要	Human apurinic/apyrimidinic endonuclease/redox effector factor 1 (APE1) is an essential DNA repair protein. Herein, we demonstrate that avidin-oriented abasic site-containing DNA strands (AP-DNA) on the surface of silica coated magnetic nanoparticles (SiMNP) can selectively respond to APE1 while resist the digestion by other nucleases. Mechanism studies have revealed that avidin may serve as an organizer protein and recruit APE1 to the DNA substrates on the nanoparticles via strong and specific interactions. Taking advantage of this newly disclosed property, we for the first time successfully displayed the intracellular activities of APE1 in living cells by fluorescence imaging. The avidin organized AP-DNA-SiMNP assembly holds great potential for enzymemediated release of drugs inside tumor cells which often contain higher levels of APE1 than normal cells.; National Natural Science Foundation of China [81571130100, 21575008, 21375004]; Beijing Natural Science Foundation [2152014]; National Natural Science Foundation of China; SCI(E); ARTICLE; 6; 45
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/474070]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhai, Junqiu,Liu, Yibin,Huang, Shan,et al. A specific DNA-nanoprobe for tracking the activities of human apurinic/apyrimidinic endonuclease 1 in living cells[J]. NUCLEIC ACIDS RESEARCH,2017.
APA	Zhai, Junqiu,Liu, Yibin,Huang, Shan,Fang, Simin,&Zhao, Meiping.(2017).A specific DNA-nanoprobe for tracking the activities of human apurinic/apyrimidinic endonuclease 1 in living cells.NUCLEIC ACIDS RESEARCH.
MLA	Zhai, Junqiu,et al."A specific DNA-nanoprobe for tracking the activities of human apurinic/apyrimidinic endonuclease 1 in living cells".NUCLEIC ACIDS RESEARCH (2017).