Constructing a Robust Fluorescent DNA-Stabilized Silver Nanocluster   Probe Module by Attaching a Duplex Moiety

doi:10.1002/chem.201701879

CORC > 北京大学 > 化学与分子工程学院

	Constructing a Robust Fluorescent DNA-Stabilized Silver Nanocluster Probe Module by Attaching a Duplex Moiety
	Lin, Ruoyun ; Tao, Guangyu ; Chen, Yang ; Chen, Mingxing ; Liu, Feng ; Li, Na
刊名	CHEMISTRY-A EUROPEAN JOURNAL
	2017
关键词	fluorescence enhancement fluorescent DNA-Ag nanoclusters fluorescent probes nanoparticles nanospecies transformation METAL NANOCLUSTERS QUANTUM DOTS NUCLEIC-ACID MULTIPLEXED ANALYSIS LABEL-FREE LIGHT-UP FLUOROPHORES GENERATION TEMPLATE MICRORNA
DOI	10.1002/chem.201701879
英文摘要	Fluorescent DNA-templated silver nanoclusters (DNA-Ag NCs) have served as excellent luminescent probes and operation units in various applications. However, the fluorescence property of DNA-Ag NCs is very sensitive to elongation or modification of the DNA template, limiting the breadth of applications. In this work, we propose a strategy for constructing a robust fluorescent DNA-Ag NCs probe module by attaching a duplex moiety to the nanocluster-bearing sequence. The fluorescence intensity of the DNA-Ag NCs can be enhanced 90-fold upon hybridization of the elongated moiety. Adenine in the linker sequence has a further enhancing effect on the fluorescence intensity, whereas thymine has a quenching effect. The transformation from a non-fluorescent species to fluorescent nanoclusters is responsible for the fluorescence enhancement with duplex formation of the elongated moiety. We hope that this design will aid future diversification of experimental designs to facilitate more applications that are currently limited by the aforementioned problems.; National Natural Science Foundation of China [21475004, 21275011, 21535006]; SCI(E); ARTICLE; 45; 10893-10900; 23
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/471534]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Lin, Ruoyun,Tao, Guangyu,Chen, Yang,et al. Constructing a Robust Fluorescent DNA-Stabilized Silver Nanocluster Probe Module by Attaching a Duplex Moiety[J]. CHEMISTRY-A EUROPEAN JOURNAL,2017.
APA	Lin, Ruoyun,Tao, Guangyu,Chen, Yang,Chen, Mingxing,Liu, Feng,&Li, Na.(2017).Constructing a Robust Fluorescent DNA-Stabilized Silver Nanocluster Probe Module by Attaching a Duplex Moiety.CHEMISTRY-A EUROPEAN JOURNAL.
MLA	Lin, Ruoyun,et al."Constructing a Robust Fluorescent DNA-Stabilized Silver Nanocluster Probe Module by Attaching a Duplex Moiety".CHEMISTRY-A EUROPEAN JOURNAL (2017).