Highly efficient enrichment of N-linked glycopeptides using a   hydrophilic covalent-organic framework

doi:10.1039/c7an01027c

CORC > 北京大学 > 化学与分子工程学院

	Highly efficient enrichment of N-linked glycopeptides using a hydrophilic covalent-organic framework
	Ma, Yu-Fang ; Yuan, Fang ; Zhang, Xiao-Hui ; Zhou, Ying-Lin ; Zhang, Xin-Xiang
刊名	ANALYST
	2017
关键词	ZIC-HILIC COMPOSITES MAGNETIC NANOPARTICLES FACILE SYNTHESIS SELECTIVE ENRICHMENT MESOPOROUS SILICA MASS-SPECTROMETRY POLYMERIZATION GLYCOSYLATION CRYSTALLINE GRAPHENE
DOI	10.1039/c7an01027c
英文摘要	The enrichment of glycopeptides plays an important role in glycoproteomics. In this paper, a covalent-organic framework called TpPa-1, synthesized by the Schiff base reaction of 1,3,5-triformylphloroglucinol and paraphenylenediamine, was first successfully utilized as a hydrophilic porous material for N-linked glycopeptide enrichment. Using this material, interference from non-glycopeptides could be efficiently eliminated, which facilitated the mass spectrometry detection of glycopeptides. By capturing N-linked glycopeptides from tryptic digests of human IgG, our method was proved to have high sensitivity at the femtomole level. And it showed superior selectivity for glycopeptides even when non-glycopeptides were 1000 times more concentrated. Due to the strong covalent bonds, this material possessed good stability and could be repeatedly used for at least 10 times. The ultra-low mass density and abundant binding sites also provided it with high binding capacity (178 mg g(-1), IgG/TpPa(-1)). Moreover, N-linked glycopeptides were easily enriched by this material from only 10 mu L human serum, which demonstrated its potential in pretreatment of complex biological samples.; National Natural Science Foundation of China [21675004, 21575005, 21275009]; SCI(E); ARTICLE; 17; 3212-3218; 142
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/470924]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Ma, Yu-Fang,Yuan, Fang,Zhang, Xiao-Hui,et al. Highly efficient enrichment of N-linked glycopeptides using a hydrophilic covalent-organic framework[J]. ANALYST,2017.
APA	Ma, Yu-Fang,Yuan, Fang,Zhang, Xiao-Hui,Zhou, Ying-Lin,&Zhang, Xin-Xiang.(2017).Highly efficient enrichment of N-linked glycopeptides using a hydrophilic covalent-organic framework.ANALYST.
MLA	Ma, Yu-Fang,et al."Highly efficient enrichment of N-linked glycopeptides using a hydrophilic covalent-organic framework".ANALYST (2017).